Expression of cytokeratins in Helicobacter pylori –associated chronic gastritis of adult patients infected with cagA+ strains: An immunohistochemical study

Table 1 Antral histology according to Sydney classification of gastritis in patients with and without Helicobacter pylori infection

Antral mucosa(Sydney classification)	H pylori+(n=57)	H pylori-(n=17)	P
Inflammatory infiltrate
0	0	1	0.000
1	19	14
2	31	2
3	7	0
Activity of inflammation
0	2	9	0.000
1	39	6
2	15	2
3	1	0
Atrophy
0	45	13	NS
1	12	4
H pylori-colonization density
0	0	17
1	19	0
2	25	0
3	13	0

H pylori+: Helicobacter pylori positive patients; H pylori -: Helicobacter pylori negative patients; NS: P > 0.05.

Table 2 Expression of CK8 in antral mucosa of patients with gastritis (H pylori positive and negative) compared to the control group

Cytokeratin 8expression	Antral mucosa epithelium
	H pylori+G		H pylori-G		CG		P
	n	%	n	%	n	%
Surface epithelium, foveolae and glandular necks
Diffuse immunoreactivity	43	75.4	14	82.4	9	90	NS
Focal immunoreactivity	14	24.6	3	17.6	1	10	NS
Without staining	0	0	0	0	0	0	NS
n (%)	57	100	17	100	10	100
Deep glands
Diffuse immunoreactivity	24	42.1	11	64.7	5	50	NS
Focal immunoreactivity	15	26.3	2	11.8	4	40	NS
Without staining	18	31.6	4	23.5	1	10	NS
n (%)	57	100	17	100	10	100

CG: control group; H pylori+G: H pylori positive patients with gastritis; H pylori-G -: H pylori negative patients with gastritis; NS: P > 0.05

Table 3 Expression of CK18 in antral mucosa of patients with gastritis (H pylori positive and negative) compared to the control group

Cytokeratin 18expression	Antral mucosa epithelium
	H pylori+G		H pylori-G		CG		P
	n	%	n	%	n	%
Surface epithelium
Diffuse immunoreactivity	32	56.1	14	82.3	5	50.0	NS
Focal immunoreactivity	24	42.1	2	11.8	3	30.0	NS
Without staining	1	1.7	1	5.9	2	20.0	0.043
n (%)	57	100	17	100	10	100
Foveolar epithelium
Diffuse immunoreactivity	31	54.4	8	47.1	1	10	0.035
Moderate/Strong	30	52.7	8	47.1	1	10	0.045
Weak	1	1.7	0	0	0	0	NS
Focal immunoreactivity	20	35.1	1	5.8	0	0	0.008
Moderate/Strong	5	8.8	0	0	0	0	NS
Weak	15	26.3	1	5.8	0	0	0.045
Without staining	6	10.5	8	47.1	9	90	<0.001
n (%)	57	100	17	100	10	100
Glandular necks
Diffuse immunoreactivity	36	63.2	13	76.5	6	60	NS
Focal immunoreactivity	18	31.6	2	11.8	2	20	NS
Without staining	3	5.2	1	5.9	2	20	NS
n (%)	57	100	17	100	10	100
Deep glands
Diffuse immunoreactivity	15	26.3	8	47.0	4	40	NS
Moderate/Strong	15	26.3	5	29.4	3	30	NS
Weak	0	0	3	17.6	1	10	0.008
Focal immunoreactivity	18	31.6	3	17.7	4	40	NS
Without staining	24	42.1	6	10.3	2	20	NS
n (%)	57	100	17	100	10	100

CG:control group; H pylori+G -:Helicobacter pylori positive patients with gastritis; H pylori-G: Helicobacter pylori negative patients with gastritis; NS: P > 0.05

Table 4 Expression of CK19 in antral mucosa of patients with gastritis (H pylori positive and negative) compared to the control group

Cytokeratin 19expression	Antral mucosa epithelium
	H pylori+G		H pylori-G		CG		P
	n	%	n	%	n	%
Surface epithelium
Expression in all cells	34	60.6	12	70.6	5	55.6	NS
Patchy staining	21	37.4	5	29.4	4	44.4
Without staining	1	2.0	0	0	0	0
n (%)	56	100	17	100	9	100
Foveolar epithelium
Expression in all cells	39	69.7	9	53.0	5	55.6	NS
Patchy staining	7	12.5	5	29.4	4	44.4	0.027
Moderate/Strong	2	3.6	2	11.8	2	22.2
Weak	5	8.9	3	17.6	2	22.2
Without staining	10	17.8	3	17.6	0	0	NS
n (%)	56	100	17	100	9	100
Glandular neck
Expression in all cells	37	58.1	9	53	6	66.7	NS
Patchy staining	10	17.8	6	25.2	3	33.3
Without staining	9	16.1	2	11.8	0	0
n (%)	56	100	17	100	9	100
Deep gland
Expression in all cells	22	39.2	6	35.2	3	33.3	NS
Patchy staining	10	17.8	5	29.5	2	22.2
Without staining	24	43.0	6	35.3	4	44.5
n (%)	56	100	17	100	9	100

CG: control group; H pylori+G: Helicobacter pylori positive patients with gastritis; H pylori-G: Helicobacter pylori negative patients with gastritis; NS: P > 0.05.

Table 5 Expression of CK20 in antral mucosa of patients with gastritis (H pylori positive and negative) compared to the control group

Cytokeratin 20expression	Antral mucosa epithelium
	H pylori+G		H pylori-G		CG		P
	n	%	n	%	n	%
Surface epithelium
Expression in all cells	37	64.9	12	70.6	9	90
Patchy staining	20	35.1	5	29.4	1	10	NS
Without staining	0	0	0	0	0	0
n (%)	57	100	17	100	10	100
Upper foveolar region
Expression in all cells	32	56.1	12	70.6	9	90	NS
Patchy staining	25	43.9	5	29.4	1	10
Moderate/Strong	20	35.1	4	23.5	1	10
Weak	5	8.8	1	5.9	0	0	0.044
Without staining	0	0	0	0	0	0
n (%)	57	100	17	100	10	100

CG:control group; H pylori+G: Helicobacter pylori positive patients with gastritis; H pylori-G: Helicobacter pylori negative patients with gastritis; NS: P > 0.05.

Table 6 Expression of CK7 in antral mucosa of patients with gastritis (H pylori positive and negative) compared to the control group

Cytokeratin 7expression	Antral mucosa epithelium
	H pylori+G		H pylori-G		CG		P
	n	%	n	%	n	%
Surface and foveolar epithelium
Weak focal
immunoreactivity	0	0	0	0	0	0	NS
Moderate focal
immunoreactivity	5	9.1	0	0	0	0	NS
<10% cells	0	0	0	0	0	0
10-20% cells	3	5.5	0	0	0	0
>20% cells	2	3.6	0	0	0	0
Without staining	50	90.9	16	100	10	100	NS
n (%)	55	100	16	100	10	100
Glandular necks and deep glands
Weak focal
immunoreactivity	6	10.9	1	6.3	3	30.0
<10% cells	3	5.5	1	6.3	3	30.0	0.01
10-20% cells	2	3.6	0	0	0	0	NS
>20% cells	1	1.8	0	0	0	0	NS
Moderate focal
immunoreactivity	38	69.1	10	62.5	7	70.0
<10% cells	10	18.3	10	62.5	7	70.0	0.002
10-20% cells	14	25.4	0	0	0	0	0.002
>20% cells	14	25.4	0	0	0	0	0.002
Without staining	11	20.0	5	31.2	0	0
n (%)	55	100	16	100	10	100

CG: control group; H pylori+G: Helicobacter pylori positive patients with gastritis; H pylori-G: Helicobacter pylori negative patients with gastritis; NS: P > 0.05.