Cyclin D1 antisense oligodexoyneucleotides inhibits growth and enhances chemosensitivity in gastric carcinoma cells

Table 1 Sequences of primers (bp)

	Primers	Length of amplification
Cyclin D1	5’-GGA TGC TGG AGG TCT GCG AGG AAC-3’	514
	5’-GAG AGG AAG CGT GTG AGG CGG TAG-3’	(332--845)
TS	5’-CAC ACT TTG GGA GAT GCA CAT ATT T -3’	208
	5’-CTT TGA AAG CAC CCT AAA CAG CCA T-3’	(853--1060)
TP	5’-ACA AGG TCA GCC TGG TCC TC-3’	353
	5’-TCC GAA CTT AAC GTC CAC CAC -3’	(491--834)
DHFR	5’-TCC ATT CCT GAG AAG AAT CGA CCT T-3’	231
	5’-CAC AAA TAG TTT AAG ATG GCC TGG G-3’	(657--887)
G3PDH	5’-TCC CTC AAG ATT GTC AGC AA-3’	309
	5’-AGA TCC ACA ACG GAT ACA TT-3’

Table 2 IC50 of 5-FU, MTX and CDDP in each group (×10⁶ mol/L)

	5-FU	MTX	CDDP
Chemotherapy Group	3.95 ± 0.92	1.27 ± 0.32	2.12 ± 0.22
SODN+Chemotherapy Group	4.43 ± 0.41	1.25 ± 0.10	1.88 ± 0.07
ASODN+Chemotherapy Group	0.98 ± 0.15 ac	0.38 ± 0.04 ac	0.52 ± 0.07 ac

^aP<0.05 vs Chemotherapy Group;

^cP<0.05 vs SODN+ Chemotherapy Group.