A microarray-based gastric carcinoma prewarning system

Table 1 Differentially expressed genes in prewarning microarray of gastric cancer.

GenBank	Number	Description of gene
Highly expressed genes in gastric cancer
1	NM_001962	Homo sapiens ephrin-A5 (EFNA5)
2	XM_017384	Homo sapiens matrix metalloproteinase 7 (MMP7)
3	NM_008610	Mus musculus matrix metalloproteinase 2 (Mmp2)
4	NM_004995	Homo sapiens matrix metalloproteinase 14 (MMP14)
5	AF093573	Bos taurus angiopoietin-1 (ang-1)
6	AF004327	Homo sapiens angiopoietin-2
7	M11730	Human tyrosine kinase-type receptor (HER2)
8	U13948	Human zinc finger/leucine zipper protein (AF10)
9	XM_049646	Homo sapiens similar to octamer-binding transcription factor 3B (OCT-3B)
10	XM_055784	Homo sapiens fibroblast growth factor 2 (basic) (FGF2)
11	XM_056035	Homo sapiens proliferating cell nuclear antigen (PCNA)
12	L24203	Homo sapiens ataxia-telangiectasia group D-associated protein
13	XM_087201	Homo sapiens similar to RED protein, IK cytokine
14	X00663	Human mRNA fragment for epidermal growth factor (EGF) receptor
15	NM_002607	Homo sapiens platelet-derived growth factor alpha polypeptide (PDGFA)
16	XM_165656	Homo sapiens matrix metalloproteinase 2 (MMP2)
17	NM_005918	Homo sapiens malate dehydrogenase 2, NAD (mitochondrial) (MDH2)
18	AF503165	Homo sapiens HUS1 checkpoint homolog (HUS1) gene
19	XM_045667	Homo sapiens antigen identified by monoclonal antibody Ki-67 (MKI67)
20	XM_050913	Homo sapiens frequently rearranged in advanced T-cell lymphomas (FRAT1)
21	XM_032866	Homo sapiens signal transducer and activator of transcription 5A (STAT5A)
22	NM_004103	Homo sapiens protein tyrosine kinase 2 beta (PTK2B)
23	XM_008355	Homo sapiens membrane protein, palmitoylated 2 (MPP2)
24	L18920	Human MAGE-2 gene exon 2, 3, 4
25	M12174	Human ras-related rho
26	NM_012333	Homo sapiens c-myc binding protein (MYCBP)
27	BC016514	Homo sapiens, similar to translocated promoter region (to activated MET oncogene)
28	NM_004324	Homo sapiens BCL-2 associated X protein (BAX)
29	Z26580	cyclin A
30	D45906	LIMK-2
31	D21255	OB-cadherin-2
32	X54925	Type I interstitial collagenase
33	X05232	Stromelysin, matrix metalloproteinase 3
34	M22612	Human pancreatic trypsin 1 (TRY1)
35	XM_055254	Homo sapiens fibronectin 1 (FN1)
36	AF081127	Danio rerio fibronectin (fn2)
37	M15796	Human cyclin protein gene
38	HSFIBEDA	Human fibronectin gene ED-A region
39	HSU66406	Human putative EPH-related PTK receptor ligand LERK-8 (Eplg8)
40	AF068846	Homo sapiens scaffold attachment factor A (SAF-A)
41	HSBTRCP	Homo sapiens mRNA for beta-transducin repeat containing protein
42	AF110763	Homo sapiens skeletal muscle LIM-protein 1 (FHL1) gene
43	HUMHO2SOS1	Human mRNA for heme oxygenase-2
44	HSHMSH16	Human mutator hMSH2 gene
45	HSEHK1	Homo sapiens mRNA for EHK-1 receptor tyrosine kinase
46	HSKLON30	Homo sapiens mRNA for unknown antigen
47	AB005047	Homo sapiens mRNA for SH3 binding protein
48	AF070561	Homo sapiens clone 24703 beta-tubulin
49	HUMCAM1V	Human vascular cell adhesion molecule 1
50	HSRNASMG	Homo sapiens mRNA for Sm protein G
51	X83228	Homo sapiens mRNA for LI-cadherin
52	AF125100	Homo sapiens HSPC039 protein
53	HSU97018	Homo sapiens echinoderm microtubule-associated protein homolog HuEMAP
54	HSU43188	Human Ets transcription factor (NERF-2)
55	HSY17392	Homo sapiens mRNA for prefoldin subunit 1
56	HSU08316	Human insulin-stimulated protein kinase 1 (ISPK-1)
57	HZNF232G2	Homo sapiens zinc finger protein ZNF232, exons2 and 3
58	HUMP53T	Human p53 cellular tumor antigen
59	J03040	Human SPARC/osteonectin
60	XM_053809	Homo sapiens similar to chondroitin sulfate proteoglycan 2 (versican)
61	L40379	Homo sapiens thyroid receptor interactor (TRIP10)
62	HSU72069	Human karyopherin beta2
63	HUMPGK2	Human phosphoglycerate kinase (pgk) mRNA, exons 2 to last
64	HSU07139	Human voltage-gated calcium channel beta subunit
65	XM_001472	Homo sapiens v-jun sarcoma virus 17 oncogene homolog (avian) (JUN)
66	AU100088	Human phosphogluconate dehydrogenase (hPGDH) gene
67	HUMKRUPZN	Human Kruppel related zinc finger protein (HTF10)
68	AF077050	Homo sapiens neuroendocrine-specific protein C homolog
69	HUMSC35A	Human splicing factor SC35
70	HUMPTPB	Homo sapiens protein tyrosine phosphatase (CIP2)
71	AF049608	Homo sapiens monocarboxylate transporter 2 (MCT2)
72	HUMHEK	Human receptor tyrosine kinase (HEK)
73	J03210	Human collagenase type IV
74	HSRAB9P40	Homo sapiens mRNA for Rab9 effector p40
75	AF184924	Homo sapiens zinc finger transcription factorBTEB2 gene
76	HUMC5A2A	Human fibrillar collagen (proa2 (V)) gene
77	HUMGAPA	Human GTPase-activating protein ras p21 (RASA)
78	HUMGLURS	Human glutamate receptor subunit (GluH1)
79	AF047715	Homo sapiens A-kinase anchoring protein (AKAP18)
80	HSU40282	Homo sapiens integrin-linked kinase (ILK)
81	HSATPF1M	Human mRNA for mitochondrial ATP synthase(F1-ATPase) alpha subunit
82	AF152485	Homo sapiens protocadherin alpha 7 short formprotein (PCDH-alpha7)
83	HSRP19	Human mRNA for 19 ku protein of signal recognition particle (SRP)
84	U17195	Homo sapiens A-kinase anchor protein (AKAP100)
85	HSU79299	Human neuronal olfactomedin-related ER localizedprotein
86	XM_037859	Human focal adhesion kinase (FAK)
87	HSU04209	Human-associated microfibrillar protein
88	D82878	Hemicentrotus pulcherrimus mRNA for p34cdc2
89	AF060515	Homo sapiens cyclin K (CPR4)
90	D21262	Human mRNA for KIAA0035 gene
91	NM_005641	Homo sapiens TATA box binding protein-associatedfactor, RNA polymerase II, 85 ku
92	HSU07550	Human chaperonin 10
93	X82153	Homo sapiens mRNA for cathepsin 0
94	HSU41766	Human metalloprotease/disintegrin/cysteine-richprotein precursor (MDC9)
95	AB017019	Homo sapiens mRNA for JKTBP2
96	HUMFNC	Human cellular fibronectin
97	U93033	Homo sapiens thyroglobulin (TG)
98	AF0304354	Homo sapiens proteoglycan 3 (PRG3) gene
99	HUMCOL3IX	Homo sapiens collagen alpha 3 type IX (COL9A3)
100	NM_002427	Homo sapiens matrix metalloproteinase 13(MMP13)
101	AF039747	Homo sapiens cadherin-10 (CDH10)
102	AF072242	Homo sapiens methyl-CpG binding protein MBD2(MBD2)
103	HSMYCC	Human c-myc oncogene
104	HSTSPM	Homo sapiens tissue specific mRNA
105	HSU64317	Human Crk-associated substrate relatedprotein Cas-L
106	HSVACM1	Homo sapiens mRNA for vasopressin activatedcalcium mobilizing receptor-like protein
107	HUMPA1V	Human pro-alpha-1 (V) collagen
108	AF059611	Homo sapiens nuclear matrix protein NRP/B (NRPB)
109	HSU004845	Human a6 (I V) collagen (COL4A6)
110	M87860	Human S-lac lectin L-14-II (LGALS2) gene
111	AF492837	Human mRNA for osteopontin
112	HSCOX7BM	Homo sapiens coxVIIb mRNA for cytochromec oxidase subunit VIIb
113	U01244	Human fibulin-1D
114	U52153	Human inwardly rectifying potassium channelKir3.2
115	S66427	RBP1=retinoblastoma binding protein 1 [human, Nalm-6 pre-B cell leukemia, mRNA, 4834 nt]
116	AF117108	Homo sapiens IGF-II mRNA-binding protein3 (IMP-3)
117	HSU49083	Human cell surface heparin binding protein HIP
118	HSU59289	Human H-cadherin
119	HSU95032	Human growth-arrest-specific protein 2
120	HSU18018	Human E1A enhancer binding protein (E1A-F)
121	HUMCGRPB	Homo sapiens (clone HSNME29) CGRP type1 receptor
122	X59543	Human mRNA for M1 subunit of ribonucleotidereductase
123	AF072810	Homo sapiens transcription factor WSTF
124	AF005068	Homo sapiens breast and ovarian cancersusceptibility protein splice variant (BRCA1)
125	HSU66197	Human fibroblast growth factor homologous factor1 (FHF-1)
126	HUMVTNR	Human cell adhesion protein (vitronectin) receptoralpha subunit
127	HSA6417	Homo sapiens mRNA for beta-tubulin foldingcofactor D
128	AF109126	Homo sapiens stromal cell-derived receptor-1 beta
129	AB030078	Homo sapiens mRNA for K-sam-II03
130	HUMMFAP	Homo sapiens extracellular matrix protein (MFAP3)gene
131	HUMCOLVA	Human alpha-2 type V collagen gene
132	HUMAAMP1X	Homo sapiens angio-associated migratory cell protein (AAMP)
133	Y08319	Homo sapiens mRNA for kinesin-2
134	HSVWFR1	Human mRNA for pre-pro-von Willebrand factor
135	S60085S2	ADMLX=putative adhesion molecule [human,mRNA, 4121 nt, segment 2 of 2]
136	HSU51334	Homo sapiens signal transducing adaptor molecule 2A (STAM2)
137	AF435957	Homo sapiens Ly-6 antigen/uPA receptor-likedomain-containing protein
138	NM_000245	Homo sapiens met proto-oncogene (hepatocytegrowth factor receptor)
139	XM_044659	Homo sapiens c-src tyrosine kinase (CSK)
140	AF061573	Homo sapiens protocadherin (PCDH8)
141	HUMMFAP	Homo sapiens extracellular matrix protein (MFAP3)gene
142	AF081535	Homo sapiens CDC45L (CDC45L)
143	HUMCA1XIA	Human alpha-1 type XI collagen (COL11A1)
144	AB016625	Homo sapiens OCTN2 gene
145	AF151899	Homo sapiens CGI-141 protein
146	HSU12535	Human epidermal growth factor receptor kinasesubstrate (Eps8)
147	HSFCRIB	Human mRNA for high affinity Fc receptor (FcRI) 慴 form’
148	HSU74628	Homo sapiens cell division control related protein (hCDCrel-1)
149	AF039564	Homo sapiens retinoblastoma binding protein (RBBP9)
150	HUMGAPA	Human GTPase-activating protein ras p21 (RASA)
151	HUMSTK2A	Human protein serine/threonine kinase stk2
152	AF144700	Homo sapiens small zinc finger-like protein (TIM13)
153	HUMTUBAK	human alpha-tubulin
154	HUMADCY	Homo sapiens adenyl cyclase-associated protein (CAP)
155	HSU89329	Human alternatively spliced microtubule-associatedprotein 2C (MAP2)
156	BC00051	Homo sapiens, Insulin-like growth factor 2
157	HSU89329	Human alternatively spliced microtubule-associated protein 2C (MAP2)
158	BC00051	Homo sapiens, insulin-like growth factor 2
159	AB000529	Homo sapiens, prostate differentiation factor
160	HSMAP01	Human microtuble-associated protein-2 (MAP-2) gene, exon 1
161	X67951	Human mRNA for proliferation-associated gene (pag)
162	M94250	Human retinoic acid inducible factor (MK) gene
163	XM_046278	Homo sapiens core promoter element binding protein (COPEB)
164	HSBM40	Human mRNA for extracellular matrix protein BM-40
165	HSU76381	Homo sapiens fibroblast growth factor (FGF-12b)
166	HSCALM2S04	Homo sapiens calmodulin (CALM2) gene, exons 3-6
167	HUMID2X	Human helix-loop-helix protein(ID-2)
168	U20758	Human osteopontin gene
169	AF152307	Homo sapiens protocadherin alpha 11(PCDH-alpha11)
170	HUMAAMP1X	Homo sapiens angio-associated migratory cell protein (AAMP)
171	HUMMXI1A	Human MXI1
172	AF143536	Homo sapiens colon cancer-associated protein Mic1(MIC1)
173	HSU70322	Human transportin (TRN)
174	HUMMNMP	Human major nuclear matrix protein
175	AF071057	mRNA differentially expressed in GC7901 and GES-1
176	AF219140	Homo sapiens gastric cancer-related protein GCYS-20
177	HSU40282	Homo sapiens integrin-linked kinase (ILK)
178	HSCA2VR	Human mRNA for pro-alpha 2 (V) collagen chain
179	HUMPECAM27	Homo sapiens platelet/endothelial cell adhesion molecule-1 (PECAM-1) gene
180	XM_165823	Homo sapiens tumor necrosis factor (TNF superfamily, member 2) (TNF)
181	D21063	Homo sapiens MCM2 minichromosome maintenance deficient 2, mitotin
182	XM_168045	Homo sapiens CD24 antigen (small cell lung carcinoma cluster 4 antigen) (CD24)
183	XM_030326	Homo sapiens CD44 antigen (CD44)
184	XM_034862	Homo sapiens interferon regulatory factor 1 (IRF1)
185	AB025106	Homo sapiens mRNA for E-cadherin
186	U73704	Homo sapiens 48 ku FKBP-associated protein FAP48
187	AF380298	Oncorhynchus mykiss interferon regulatory factor 1 gene, promoter region and partial sequence
188	L24203	Homo sapiens ataxia-telangiectasia group D-associated protein
189	D45906	Homo sapiens mRNA for LIMK-2
190	D21255	Human mRNA for OB-cadherin-2
191	X54925	Homo sapiens mRNA for type I interstitial collagenase
192	X05232	Human mRNA for stromelysin, matrix metalloproteinase 3
193	M22612	Human pancreatic trypsin 1 (TRY1)
194	HUMGOS8PPC	Human helix-loop-helix basic phosphoprotein (GOS8)
195	HUMTHBS3	Homo sapiens thrombospondin 3 (THBS3) gene
196	HSVECAD	Homo sapiens VE-cadherin
197	HSBTRCP	Homo sapiens mRNA for beta-transducin repeat containing protein
198	HUMPROFII	Human profilin II
199	HSCALT	Homo sapiens mRNA for caltractin
200	AF091214	Homo sapiens WRN (WRN)
201	AF070561	Homo sapiens clone 24703 beta-tubulin
202	HUMCD14MCA	Human monocyte antigen CD14 (CD14)
203	HUMCAM1V	Human vascular cell adhesion molecule 1
204	AF032900	Homo sapiens timing protein CLK-1
205	AF070561	Homo sapiens clone 24703 beta-tubulin
206	HSUPUE	Homo sapiens mRNA for unknown protein of uterine endometrium
207	HUMIL8RB	Homo sapiens interleukin 8 receptor beta (IL8RB)
208	HSERK3	Homo sapiens ERK3
209	AF208045	Homo sapiens breast cancer-associated antigen BRCAA1 (BRCAA1)
210	AF081259	Homo sapiens testis-specific chromodomain Y-like protein (CDYL)
211	AB022918	Homo sapiens mRNA for alpha2,3-sialyltransferaseST3Gal VI
212	AF057036	Homo sapiens acetylcholinesterase collagen-like tail subunit (COLQ)
213	AF152497	Homo sapiens protocadherin beta 4 (PCDH-beta4)
214	M86752	Stress-induced phosphoprotein 1
215	L04270	TNF C receptor
216	AF009674	Axin 1
Highly expressed genes in precancerous lesions
1	HSU72621	Human LOT1
2	HUMNMOR	Human NAD(P)H: menadione oxidoreductase
3	AF009227	Homo sapiens gamma-heregulin
4	HSU44839	Human putative ubiquitin C-terminal hydrolase(UHX1)
5	HUMAAE	Homo sapiens dbpB-like protein
6	HSU08316	Human insulin-stimulated protein kinase 1 (ISPK-1)
7	HUMCD3621	Human antigen CD36 (clone 21)
8	Z11899	Homo sapiens OTF3 mRNA for encoding octamer binding protein 3B
9	XM_003226	Homo sapiens vasoactive intestinal peptide receptor 1 (VIPR1)
10	HUMPAI2B	Human plasminogen activator inhibitor 2 (PAI-2)
11	HUMACTIIA	Human activin type II receptor
12	M93718	Human nitric oxide synthase
13	HSU46837	Human RNA polymerase II holoenzyme componentSRB7 (SRB7)
14	HUMPCNA	Human proliferating cell nuclear antigen (PCNA) gene
15	HSPCAR	Human mRNA for calcium dependent protease(small subunit)
16	HSU12140	Human tyrosine kinase receptor p145TRK-B(TRK-B)
17	HSTOP2A10	Homo sapiens topoisomerase II alpha (TOP2A) gene,exons 34 and 35
18	HUMYWXD703	Homo sapiens ADP/ATP carrier protein (ANT-2)gene
19	HUMKGF	Human keratinocyte growth factor
20	HS40KDAP	Homo sapiens 40 ku protein kinase related to ratERK2
21	HUMPAFAA	Human mRNA for platelet activating factoracetylhydrolase IB gamma-subunit
22	HUMLPL	Human lipoprotein lipase
23	HUMMYLCC	Human smooth muscle myosin alkali light chain (MLC 1sm)
24	HSU10564	Human CDK tyrosine 15-kinase WEE1Hu (Wee1Hu)
25	AF022655	Homo sapiens cep250 centrosome associated protein
26	D49737	Homo sapiens mRNA for cytochrome b large subunitof complex II
27	HUMCD53GLY	Human CD53 glycoprotein
28	L02867	Homo sapiens 62 ku paraneoplastic antigen
29	HUMCALBETB	Human voltage-dependent calcium channel beta-1subunit
30	HUMEPSURAN	Human surface antigen
31	AB020647	Homo sapiens mRNA for KIAA0840 protein
32	HSU88966	Human protein rapamycin associated protein(FRAP2) gene
33	HUMHGLUT1	Human mRNA for glutamate transporter
34	U70663	Human zinc finger transcription factor hEZF(EZF)
35	HSPTS1R	Homo sapiens mRNA for peroxisomal targetingsignal 1 (SKL type) receptor
36	HSU61276	Human transmembrane protein Jagged 1 (HJ1)
37	HUMMYONM	Human nonmuscle myosin heavy chain (NMHC)
38	AF016270	Homo sapiens thyroid hormone receptor coactivating protein
39	HSU66243	Human p38 gamma MAP Kinase
40	HSU41766	Human metalloprotease/disintegrin/cysteine-richprotein precursor (MDC9)
41	HUMELF2	Human translational initiation factor 2 beta subunit(elF-2-beta)
42	HUMCYCAA	Human somatic cytochrome c (HCS) gene
43	NM_013217	Homo sapiens gene for AF-6
44	AB017642	Homo sapiens mRNA for oxidative-stress responsive 1
45	AF110956	Homo sapiens SUMO-1 activating enzyme subunit 1
		(SAE1)
46	HUMALR	Human aldehyde reductase
47	HUMATPSAS	Human gene for ATP synthase alpha subunit (exon1 to 12)
48	AF052497	Homo sapiens clone B18
49	AB000889	Homo sapiens mRNA for phosphatidic acidphosphatase 2b
50	HUMTPARN	Homo sapiens mRNA for tissue plasminogen activator.
51	AF006082	Homo sapiens actin-related protein Arp2 (ARP2)
52	HSU21090	Human DNA polymerase delta small subunit
53	HUMVENHK1	Human voltage-gated potassium channel (HK1)
54	HUMVTNR	Human cell adhesion protein (vitronectin) receptoralpha subunit
55	AF091242	Homo sapiens ATP sulfurylase/APS kinase 2
56	HUMIGFBP1	Human insulin-like growth factor binding protein-1 (IGFBP1) gene
57	AF047439	Homo sapiens unknown
58	AF117386	Homo sapiens ubiquitin-specific protease (UBP)
59	AF092129	Homo sapiens guanine nucleotide binding protein gamma-3 subunit
60	HUMCOXIV	Human cytochrome c oxidase COX subunit IV(COX IV)
61	J05412	Human regenerating protein (reg) gene
62	AF054162	Gccys-1, mRNA differentially expressed betweenGC7901 and GES-1
63	AF054163	Gccys-2, mRNA differentially expressed betweenGC7901 and GES-1
64	AF054164	Gccys-3,mRNA differentially expressed betweenGC7901 and GES-1
65	AF054165	Gccys-4, mRNA differentially expressed betweenGC7901 and GES-1
66	AF054166	Gccys-5, mRNA differentially expressed betweenGC7901 and GES-1
67	AF054167	Gccys-6, mRNA differentially expressed betweenGC7901 and GES-1
68	NM_003542	Homo sapiens H4 histone family, member G(H4FG)
69	XM_032781	Homo sapiens tubulin, gamma 1 (TUBG1)
70	XM_083852	Homo sapiens ribonucleotide reductase M1polypeptide(RRM1)
71	HSU51586	Human siah binding protein 1 (siahBP1)
72	X55181	Human ETS2 gene
73	NM_004526	Homo sapiens MCM2 minichromosomemaintenance deficient 2, mitotin (MCM2)
74	XM_040900	Homo sapiens MAP/microtubule affinity-regulatingkinase 3 (MARK3)
75	XM_083852	Homo sapiens ribonucleotide reductase M1polypeptide(RRM1)
76	NM_012145	Homo sapiens deoxythymidylate kinase(thymidylate kinase) (DTYMK)
77	X59543	Ribonucleotide reductase M1 polypeptide
78	M74542	Human aldehyde dehydrogenase type III (ALDHIII)
79	M61855	Human cytochrome P4502C9 (CYP2C9)
80	S37730	Homo sapiens insulin-like growth factor bindingprotein-2
81	AB015982	Homo sapiens EPK2 mRNA for serine/threoninekinase
82	X67951	Human mRNA for proliferation-associated gene(pag)
83	AF127506	Homo sapiens adenomatosis polyposis coli tumorsuppressor (APC) gene
84	HT880	Human Gastric mucin 6
85	M63154	Gastric intrinsic factor
Highly expressed genes in normal gastric mucous
1	X05997	Human mRNA for gastric Lipase
2	U75272	Human gastricsin
3	M63154	Human intrinsic factor
4	AF043909	Homo sapiens gastric mucin (MUC5AC)
5	L07518	Homo sapiens mucin
6	M61853	Human cytochrome p4502C18 (CYP2C18)
7	M10942	Human metallothionein-Ie gene (hMT-Ie)
8	L15533	Homo sapiens pancreatitis-associated protein (PAP)gene
9	Z49107	Homo sapiens galectin
10	U52191	Human SMCY (H-Y)
11	NM_005522	Homo sapiens homeo box A1 (HOXA1)
12	M57732	Human hepatic nuclear factor 1 (TCF1)
13	X59770	Homo sapiens IL-1R2 mRNA for type II interleukin-1 receptor
14	X76223	Homo sapiens MAL gene exon 4
15	U05259	Human MB-1 gene
16	XM_052013	Homo sapiens polymeric immunoglobulin receptor(PIGR)
17	U90065	Human potassium channel KCNO1
18	M55422	Human Krueppel-related zinc finger protein (H-plk)
19	S78825	Id1, transcription regulator helix-loop-helix protein
20	U19948	Human protein disulfide isomerase (PDIp)
21	U43522	Human cell adhesion kinase beta (CAKbeta)
22	U12139	Human alphal (XI) collagen (COL11A1) gene, 5region and exon 1
23	M14539	Human factor XIII subunit
24	X65614	Homo sapiens mRNA for calcium-binding proteinS100P
25	AF000560	Homo sapiens TTF-I interacting peptide 20
26	AF002224	Homo sapiens Angelman Syndrome Gene, E6-APubiquitin protein ligase 3A
27	U57096	Human janus kinase 3 (Jak3)
28	U42600	Human calcium-activated potassium channel betasubunit
29	NM_017406	cAMP responsive element binding protein-like 1
30	U04806	Human FLT3/FLK2 ligand
31	D84361	Human p52 and p64 isoforms of N-Shc
32	Z30425	Homo sapiens orphan nuclear hormone receptor
33	M16364	Human creatine kinase-B
34	X96924	Homo sapiens encoding mitochondrial citratetransport protein
35	HSNM23H1	Homo sapiens nm23H1 gene
36	NM_014792	Homo sapiens KIAA0125 gene product (KIAA0125)
37	M34041	Human alpha-2-adrenergic receptor (aipha-2 c2) gene
38	XM_002444	Homo sapiens serine threonine kinase 39 (Stk39)
39	NM_001690	Homo sapiens ATPase, H+ transporting, lysosomal70 ku, V1 subunitA
40	L12398	Human sapiens dopamine receptor D4 (DRD4)
41	L76465	Homo sapiens NAD+ dependent 15hydroxyprostaglandin dehydrogenase (PGDH)
42	U57094	Human small GTP-binding protein
43	Z14978	Homo sapiens mRNA for actin-related protein
44	X53961	Human lactotransferrin
45	M62628	Human alpha-1 Ig germline C-region membrane-coding region
46	M84526	Human adipsin/complement factor D
47	X04391	Human lymphocyte glycoprotein T1/Leu-1
48	X044533	Homo sapiens sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) andshort cytoplasmic domain, (semaphoring) 4B(SEMA4B)
49	AF071054	Gcys-11, mRNA differentially expressed in cell linesGC7901 and GES-1
50	AF063015	Homo sapiens cell division protein
51	AF071056	Gcys-17, mRNA differentially expressed in cell lines GC7901 and GES-1
52	AF071058	Gcys-15, mRNA differentially expressed in cell lines GC7901 and GES-1
53	NM_001730	Homo sapiens Kruppel-like factor 5 (intestinal)(KLF5), mRNA
54	AB047278	Arabidopsis thaliana AtNdr 1 mRNA for Ndr kinase
55	XM_061005	Homo sapiens similar to Mucin 2 precursor(Intestinal mucin2)
56	HUM20D9	Human gene for 2-oxoglutarate dehydrogenase
57	HSCDC2	Human CDC2 gene involved in cell cycle control
58	AF202051	Homo sapiens NM23-H8 (NME8)
59	NM_005423	Homo sapiens trefoil factor 2 (spasmolytic protein 1) (TFF2)
60	D50419	Homo sapiens OTK18
61	HSU88870	Human cell division control-related protein 2b(hcdcrel2b)
62	NM_031942	Homo sapiens cell division cycle associated 7(CDCA7)
63	HSU09716	Human mannose-specific lectin (MR60)
64	HSU14394	Human tissue inhibitor of metalloproteinases-3
65	Z48314	Apomucin
66	M63154	Gastric intrinsic factor
67	J05412	Regenerating protein
68	M57732	Hepatic nuclear factor 1
69	U70663	Kruppel-like factor 4
70	AB002559	Syntaxin binding protein 2
71	U80226	GABA transaminase
72	U05259	CD79A
73	X04391	CD5
74	U60800	CD100
75	M74542	Aldehyde dehydrogenase 3
76	X66839	Carbonic anhydrase IX
77	L00972	Cystathionine-beta-synthase
78	L41688	UDP-galactose-4 epimerase
79	J03915	Chromogranin A
80	S76942	Dopamine receptor D4
81	D14695	Herp
82	D50915	D50915
83	D86961	HMGIC fusion partner-like 2
84	X96924	Mitochondrial citrate transporter
85	M16364	Creatine kinase, brain
86	M14539	Factor XIII precursor
87	U19948	Protein disulfide isomerase
88	X65614	S100 calcium binding protein P
Highly expressed genes associated with metastasis
1	NM_004994	Homo sapiens matrix metalloproteinase 9(gelatinase B, 92 ku type IV collagenase) (MMP9)
2	XM_053256	Homo sapiens mucin 1, transmembrane (MUC1)
3	XM_010702	Homo sapiens cathepsin K (pycnodysostosis) (CTSK)
4	NM_002628	Homo sapiens profiling 2 (PFN2), transcript variant 2
5	NM_002128	Homo sapiens high-mobility group protein 1 (HMG1)
6	M28130	Human interleukin 8 (IL8) gene
7	S3488	Metastasis-associated gene (human, highlymetastatic lung cell subline Anip)
8	NM_005231	Homo sapiens ems1 sequence, transcript variant 1
9	XM_059020	Homo sapiens similar to GPI-anchored metastasis-associated protein homolog
10	NP_571483	Vascular endothelial growth factor (VEGF)
11	I56986	OPN-a-human (fragment)
12	AAG31602	CD44 isoform v3-v6
13	AF018733	92 ku type IV collagenase precursor (matrixmetalloproteinase-9) (MMP-9)
14	AF00196	Octamer-binding transcription factor 2 (OTF-2)
15	XM_055254	Homo sapiens fibronectin 1 (FN1)
Risk factor genes
1	V01555	Epstein-Barr virus (EBV) genome, strain B95-8
2	AF275307	H pylori plasmid pHPM8 (cagA)
3	AF275307	H pylori plasmid vacA
4	AF275307	H pylori plasmid Urase
5	AF431736	Human herpesvirus 1 strain KOS ICPO gene
6	Z86099	Herpes simplex virus type 2 (strain HG52)
7	AF477385	Human papillomavirus type 16 E7 gene
8	AX742207	Human hepatitis virus 11 type

Table 2 Gene expression threshold for distinguishing three kinds of gastric mucosa.

Gene classification	Gastric cancer tissue (GC/N)	Precancerous lesion (PC/N)	Normal gastric mucosa (N*/GC or N*/PC)
216 genes associated with gastric cancer	6.01±2.40	1.18 ±0.47	< 0.75
85 genes associated with precancerous lesions	1.32±0.53	4.86±1.94	2.54±0.41
88 genes associated with normal mucosa	1.31±0.54	2.50±0.75	5.42±2.17
15 genes associated with metastasis of gastric cancer	5.81±2.32 (M)2.32±1.19 (N1)	1.13±0.58	0.65±0.35
8 genes associated with risk factors		>2.0

Specification: The above data indicate the relative expression levels between GC/N, PC/N, N/PC and N/GC mean ratio and minimum values. M: Metastasis; N1: No metastasis. GC: Gastric cancer; PC: Precancerous lesion; N: Normal mucosa. N^*: Selected gene expression levels in normal gastric mucosa.