Presence of CCK-A, B receptors and effect of gastrin and cholecystokinin on growth of pancreatobiliary cancer cell lines

Table 1 Characteristics of pancreatic cancer and periampullary cancer cell lines.

Cell line	In vivo			In vitro
	Origin	Degree ofdifferentiation	Nodal status	Growthcharacteristics	Viability	Doubling time	Cellmorphology
SNU-245	CBD	Well	0/9	Ad	85	54	P
SNU-308	GB	Moderately	-	Ad	88	4848	P
SNU-478	AoV	Poorly	0/8	Ad	83	52	P/S
SNU-869	AoV	Well	5/10	Ad	91	48	P
SNU-1079	IHD	Moderately	-	Ad	89	72	Ple
SNU-1196	HDB	Moderately	0/1	Ad	94	48	Spindle to P
SNU-213	Pancreas	Moderately	1/8	Ad	89	48	P
SNU-324	Pancreas	Poorly	0/15	Ad+Fl	95	52	P/S
SNU-410	Pancreas	Poorly	-	Ad	92	72	P

CBD: common bile duct, GB: gallbladder, AoV: ampulla of Vater, Ad: adherent, Fl: floating, P: polygonal, S: spherical, Ple: pleomorphic.

Table 2 Effect of gastrin and L-365,260 on periampullary cancer cell lines.

Cell line	Origin	Maximum response to hormones and hormone concentration
		Gastrin			L-365, 260
		Response (%)	Concentration (mol/L)	P-value	Response (%)	Concentration (mol/L)	P-value
SNU-245	CBD	108	10-7	NS	98	10-8	NS
SNU-308	GB	127	10-9	0.038	96	10-9	NS
SNU-478	AoV	121	10-9	0.047	94	10-9	NS
SNU-869	AoV	107	10-6	NS	103	10-7	NS
SNU-1079	IHD	107	10-9	NS	104	10-11	NS
SNU-1196	HDB	104	10-8	NS	93	10-7	NS
SNU-213	Pancreas	105	10-10	NS	97	10-8	NS
SNU-324	Pancreas	112	10-11	NS	97	10-9	NS
SNU-410	Pancreas	115	10-12	NS	98	10-8	NS
MIAPaCa-2	Pancreas	106	10-10	NS	95	10-9	NS
PANC-1	Pancreas	101	10-7	NS	94	10-8	NS

Table 3 Effect of CCK and L-364,718 on periampullary cancer cell lines.

Cell line	Origin	Maximum response to hormones and hormone concentration
		CCK			L-364, 718
		Response (%)	Concentration (mol/L)	P-value	Response (%)	Concentration (mol/L)	P-value
SNU-245	CBD	110	10-12	NS	96	10-10	NS
SNU-308	GB	109	10-7	NS	94	10-8	NS
SNU-478	AoV	123	10-9	0.043	93	10-9	NS
SNU-869	AoV	107	10-10	NS	97	10-10	NS
SNU-1079	IHD	113	10-9	NS	101	10-8	NS
SNU-1196	HDB	109	10-8	NS	94	10-8	NS
SNU-213	Pancreas	106	10-7	NS	98	10-6	NS
SNU-324	Pancreas	116	10-12	NS	96	10-10	NS
SNU-410	Pancreas	113	10-9	NS	94	10-8	NS
MIAPaCa-2	Pancreas	108	10-10	NS	97	10-9	NS
PANC-1	Pancreas	105	10-8	NS	98	10-8	NS