Polymorphisms of uridine-diphosphoglucuronosyltransferase 1A7 gene in Taiwan Chinese

Table 1 Primers used for PCR and sequencing of UGT1A7 gene.

Region		Primer		Target size (bp)
		Name	Sequence
Promoter	PCR:	U7F1	5’TGAATGAATAAGTACACGCC3’	439
		U7R1	5’ATAGAGAAAATGCACTTCGC3’
	Sequencing:	U7F1	5’TGAATGAATAAGTACACGCC3’
Exon 1	PCR:	U7F1	5’TGAATGAATAAGTACACGCC3’	779
		U7R2	5’TAGGGGCAAAATAAATGTTC3’
	Sequencing:	U7R2	5’TAGGGGCAAAATAAATGTTC3’
	PCR:	U7F2	5’ATACACTCTGGAGGATCAGG3’	774
		U7R3	5’GCTACCCAACAATTAAGTGA3’
	Sequencing:	U7F3	5’TGTCCCCAGACTTCTCTTAG3’

bp: base pair.

Table 2 Frequencies of UGT1A7 genotypes in 112 Taiwan Chinese.

UGTIA7	Number (%)
*1/*1	36 (32.1)
*1/*2	40 (35.7)
*1/*3	17 (15.2)
*2/*2	5 (4.5)
*2/*3	11 (9.8)
*3/*3	3 (2.7)

Table 3 Allele frequencies of UGT1A7 gene among different ethnic groups.

UGT1A7 alleles	Taiwan Chinese (224 chromosomes)	Caucasians (288 chromosomes)	Japanese (206 chromosomes)	P value (by χ2 test)
UGT1A7*1	0.576	0.358	0.593	<0.001
				0.73
UGT1A7*2	0.272	0.264	0.153	0.93
				0.004
UGT1A7*3	0.152	0.361	0.255	<0.001
				0.008

Table 4 Allele frequency of UGT1A7*3 in subjects carrying different UGT1A1 genotypes.

UGT1A1 gene	Number	UGT1A7 gene						Allele frequency of UGT1A7*3	Relative risk(95% CI)
		*1/*1	*1/*2	*1/*3	*2/*2	*2/*3	*3/*3
Wild type	246	114	108	9	15	0	0	0.018	1
A(TA)6TAA/A(TA)7TAA	38	13	12	7	1	5	0	0.158	8.78b
									(2.70-28.54)
A(TA)7TAA/A(TA)7TAA	31	13	5	9	1	0	3	0.242	13.44b
									(4.41-40.99)
211G/211A	90	6	10	48	0	22	4	0.433	24.06b
									(9.31-62.31)
211A/211A	100	0	0	16	0	0	84	0.92	51.11b
									(20.24-128.80)

CI: confidence interval,

^bP<0.001 vs wild type.

Table 5 Natural or mutagenesis primers, restriction enzymes and the results for UGT1A7 variations.

Position (cDNA)	Primers	Sequence	Restriction enzyme	Result (bp)
-57 T→G	U7F1	5’ TGAATGAATAAGTACACGCC	HpyCH4IV	N2	439
	U7R1	5’ ATAGAGAAAATGCACTTCGC3’		V3	57+382
33 C→A	1stPCR
	U7F1	5’ TGAATGAATAAGTACACGCC
	U7R2	5’ TAGGGGCAAAAAATGTTC3’
	2ndPCR
	U7-33F1	5’ GGGTGGACTGGCCTCCTG3’1	Taq I	N	115
	U7-33R	5’ ACTGCATGGTGAACCAGTCG		V	19+96
387 T→G	1stPCR
	U7F1	5’ TGAATGAATAAGTACACGCC
	U7R2	5’ TAGGGGCAAAATAAATGTTC
	2ndPCR
	U7-387F	5’ AAATTGCAGGAGTTCTTA3’1	Afl II	N	159
	U7-387R	5’ TGGCAAAATATTCCCCGC3’		V	140+19
622 T→C	U7F3	5’ TGTCCCCAGACTTCTCTTAG	Rsa I	N	447
	U7R3	5’ GCTACCCAACAATTAAGTGA		V	54+393

bp: base pair,

¹mutagenesis site,

²N: digestion result of wild type,

³V: digestion result of variant.