Phagocytic and oxidative burst activity of neutrophils in the end stage of liver cirrhosis

Table 1 Clinical characteristics of patients with liver cirrhosis (mean±SD)

	Child–Pugh B	Child–Pugh C
Age, year	43±12	46±13
Male/female	15/10	15/5
Albumin, g/dL	3.2±0.3	2.8±0.6
AST/ALT, IU/mL	98±65/87±98	110±45/97±45
Bilirubin, mg/dL	4.5±3.8	9.7±6.5

Table 2 Phagocytic and oxidative burst activity of neutrophils, level of soluble form of adhesive molecules and TNFα in liver cirrhosis patients (mean±SD)

	Liver cirrhosis			Healthy
	Total	Child–Pugh B	Child–Pugh C
Oxidative burst
Percentage of neutrophils with oxidative burst after PMA stimulation (%)	98.3±1.2	98.4±1.5	98.2±1.6	98.8±2.8
MIF oxidative burst after PMA stimulation	42.7±14.6b	44.3±10.1b	41.1±12.6b	50.2±13.3
Percentage of neutrophils with oxidative burst after E. coli stimulation (%)	94.0±4.8	93.0±4.1	94.0±3.8	92.2±3.7
MIF oxidative burst after E. coli stimulation	24.7±7.1b	25.1±8.1b	22.2±5.1b	29.7±6.6
Phagocytic activity
Percentage of neutrophils phagocytizing of E. coli	93.0±3.3	92.9±4.3	93.8±2.3	92.5±4.3
MIF phagocytosis E. coli	20.0±3.9	19.8±4.1	21.0±2.8	19.0±5.8
Soluble form of adhesion molecules and TNFα
sICAM-1 (ng/mL)	852±331b	788±343b	892±293b	254±74
sVCAM-1 (ng/mL)	2 937±1 591b	2 637±1 291b	3 297±1 490b	510±248
sP-selectin (ng/mL)	101±180b	98±140b	104±162b	124±58
sE-selectin (ng/mL)	136±89b	120±68b	148±58b	49±21
sL-selectin (ng/mL)	1 209±364	1 206±314	1 211±344	1 209±291
TNFα (pg/mL)	2.94±1.43b	2.64±1.33b	2.99±1.02b	1.58±0.22

^bP<0.01 vs healthy subjects (Mann–Whitney U test).

Table 3 Correlation (r) of phagocytic activity of neutrophils with their oxidative burst level and biochemical tests of liver dysfunction (Spearman test)

Correlations	r	P
MIF oxidative burst neutrophils after PMA stimulation with ALT level	-035	0.05
MIF phagocytizing E. coli with prothrombin time	-0.47	0.03
Percentage of neutrophils phagocytizing E. coli with leukocyte number	0.35	0.05
Percentage of oxidative burst neutrophils after E. coli stimulation with MIF phagocytosis E. coli	0.37	0.05