Small intestinal submucosa improves islet survival and function during in vitro culture

Table 1 Recovery of rat islets and enrichment factor of nucleosomes after being cultured for 7 and 14 d (n = 6, mean±SE)

Parameter	Group	7 d (%)	14 d (%)
Recovery
	Standard culture	67.1±2.6	42.2±1.5
	SIS culture	95.8±1.0a	90.8±1.5a
Enrichment factor
	Standard culture	1.98±0.06	2.90±0.23
	SIS culture	1.08±0.02a	1.12±0.09a

^aP<0.05 vs standard culture.

Table 2 Stimulated insulin secretion of rat islets cultured for 7 and 14 d during static incubation assay (n = 6, mean±SD)

Group	T (d)	Lowglucosesolution(2.7 mmol/L)	Highglucosesolution(16.7 mmol/L)	Highglucosesolution+50 μmol/L IBMX	SI1
Standard culture	7	9.1±0.3	18.3±1.1	51.3±3.4	5.7±0.4
SIS culture	7	7.8±0.6	21.8±1.9	75.8±2.1a	10.2±1.2a
Standard culture	14	5.9±0.4	11.8±1.1	20.5±1.8	3.6±0.4
SIS culture	14	7.1±0.4	20.7±1.1a	66.9±3.7a	9.5±0.2a

¹SI (stimulation index)=insulin secretion during incubation with 16.7 mmol/L high glucose solution+50 μmol/L IBMX/insulin secretion during incubation with 2.7 mmol/L low glucose solution.

^aP<0.05 vs standard culture.