Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109

Table 1 Apoptosis rate and ratio of differentiated cells (D)/proliferating cells (P) in each group

	E1 group	C1 group	E2 group	C2 group
Apoptosis rate	16%a	3.00%	54%c	4.00%
Ratio of D/P	1.7 (63/37)e	0.2 (17/83)	2.3 (70/30)g	0.16 (14/86)

Apoptosis rate:

^aP<0.05 vs C1 and E2 groups;

^cP<0.05 vs C2 group. Ratio of D/P:^eP<0.05 vs C1,

^gP<0.05 vs C2.

Table 2 Comparison of signal scanning values on NCM between E1 group and C1 group (mean±SD)

Groups	c-myc mRNA	Wt p53 mRNA	iNOS mRNA	EGFR-IR
E1 group	3.38 ± 0.99	2.74 ± 0.83	4.52 ± 0.74	2.37 ± 1.05
C1 group	5.18 ± 1.39	0.38 ± 0.27	2.63 ± 0.13	4.38 ± 0.48

P < 0.05, E1 vs C1 for each signal.

Table 3 Comparison of signal scanning values on NCM between E2 group and C2 group (mean±SD)

Groups	bcl-2	c-myc	iNOS	Wt p53	Fas/FasL-I
	mRNA	mRNA	mRNA	mRNA	R
E2 group	1.36 ± 0.54	1.72 ± 0.76	5.47 ± 0.35	4.76 ± 1.28	2.69 ± 0.73
C2 group	3.49 ± 1.53	5.22 ± 1.44	2.65 ± 1.22	0.42 ± 0.28	4.17 ± 0.92

P < 0.05, E2 vs C2 for each signal.

Table 4 Comparison of total integration of signal intensities for p38-IR, caspase-3-IR and NOS activity between E2 and C2 groups

Group	p38-IR	Caspase-3 IR	NOS activity
E2 group	184	348	302
C2 group	122	207	138

P < 0.05, E2 vs C2 for each signal.