Neutrophil engagement and septic challenge in acute experimental pancreatitis in rats

Table 1 WBC and PMN cells count (/µL, mean±SD)

Group	WBC after 5 h	WBC after 9 h	PMN after 5 h	PMN after 9 h
	(/µL)	(/µL)	(/µL)	(/µL)
Control	5 120  ± 1 220	5 597 ± 1 447	1230 ± 490	1 434 ± 569
AP	2 204a± 705a	4 225 ±583	614a ± 229a	1 286 ± 598
AP+MoAb	3 942c± 493c	4 037 ±1 778	2 315c±850c	1 120 ± 705
AP+LPS	1 678  ± 259	6 762c±580c	1 047 ± 560	5 146c±1 124c
AP+MoAb	4 087  ± 259	5 450 ± 580	1 830 ± 560	3 250 ± 1 124
+LPS
MoAb	6 587±1 003	3 887 ± 1 612	2 200 ± 502	990 ± 307
LPS	3 771a±1 510a	4 100 ± 1 824	2 892a±1 372a	2 959a±1 553a

^aP<0.05 vs control group;

^cP<0.05 vs AP group.

Table 2 SH compound and MDA concentration within pancreatic homogenate (nmol/mg protein, mean±SD)

Group	SH after 5 h	H after 9 h	MDA after 5 h	MDA after 9 h
Control	60.3 ± 7.3	64.7 ± 8.3	0.21 ± 0.02	0.22 ± 0.03
AP	40.2a ± 8.5a	43.5a ± 4.3a	0.38a ± 0.07a	0.39a ± 0.03a
AP+MoAb	40.0 ± 6.5	46.7 ± 1.4	0.25c ± 0.06c	0.26c ± 0.03c
AP+LPS	40.0±6.8	41.1 ± 8.2	0.39 ± 0.07	0.41 ± 0.1
AP+MoAb	39.0 ± 6.7	40.4 ± 5.0	0.37 ± 0.08	0.35 ± 0.2
+LPS
MoAb	63.4 ± 8.6	59.4 ± 9.0	0.2 ± 0.03	0.21 ± 0.04
LPS	52.0 ± 9.1	48.6 ± 4.7	0.28 ± 0.04	0.35 ± 0.05

^aP<0.05 vs control group;

^cP<0.05 vs AP group.

Table 3 Plasma α-amylase and lipase activity (U/L, mean ± SD)

Group	Α-Amylase	Α-Amylase	Lipase	Lipase
	after 5 h	after 9 h	after 5 h	after 9 h
Control	690 ± 120	743 ± 156	6 ± 8	8 ± 9
AP	8 286a ± 143a	5 908a ± 640a	2 140a ± 1 123a	929a ± 252a
AP+MoAb	5 119 ± 2 487	2 293c ± 1 739c	1 704 ± 731	342c ± 178c
AP+LPS	9 310 ± 7 552	4 001 ± 1 638	2 573 ± 2 133	1 257 ± 164
AP+MoAb	12 699c ± 4 978c	2 719c ± 634c	3 470 ± 1 832	305c ± 65c
+LPS
MoAb	696 ± 151	582 ± 273	21 ± 12	8 ± 4
LPS	584 ± 182	332 ± 93	11 ± 8	8 ± 4

^aP<0.05 vs control group;

^cP<0.05 vs AP group.

Table 4 Plasma IL-6 concentration (pg/mL, mean ± SD)

Group	IL-6 after 5 h	IL-6 after 9 h
AP	0.6 ± 2.0	0.0 ± 0.0
AP+MoAb	4.7 ± 7.0	0.0 ± 0.0
AP+LPS	982.0c ± 749.0c	10.0 ± 20.0
AP+MoAb+LPS	1 561.0c ± 493.0c	0.0 ± 0.0
Control	0.2 ± 0.3	0.1 ± 0.3
MoAb	0.0 ± 0.0	0.0 ± 0.0
LPS	941.0a ± 756.0a	2.0 ± 4.0

^aP<0.05 vs control group;

^cP<0.05 vs AP group.

Table 5 Microscopic findings within the pancreatic gland (mean ± SD)

Houvs	Edema		Vacuolization		Infiltration		Necrosis
	5	9	5	9	5	9	5	9
Group 1	0.00	0.00	0.00	0.00	1.1	1.21	0.00	0.00
Control	±0.00	±0.00	±0.00	±0.00	±0.9	±1.03	±0.00	±0.00
Group 2	1.91a	0.50	2.50a	1.00	7.40a	14.60a	0.17	0.25
AP	±0.90a	±0.57	±0.90a	±1.41	±5.29a	±2.37a	±0.38	±0.50
Group 3	2.28	1.50	1.71	1.50	3.87c	10.95	0.00	0.00
AP+MoAb CD11b	±0.75	±1.00	±0.4 8	±1.00	±1.69c	±0.92	±0.00	±0.00
Group 4	1.28	2.00	1.57	1.25	10.94	17.92	0.14	0.00
AP+LPS	±1.25	±1.15	±1.39	±1.25	±3.02	±5.98	±0.37	±0.00
Group 5	2.50	1.00	2.00	1.00	10.40	15.10	0.00	0.00
AP+MoAbCD11b +LPS	±1.00	±0.00	±1.15	±0.00	±8.30	±6.46	±0.00	±0.00
Group 6	0.25	0.00	0.00	0.00	0.80	0.25	0.00	0.00
Control+MoAb CD11b	±0.50	±0.00	±0.00	±0.00	±1.04	±0.30	±0.00	±0.00
Group 7	0.00	0.00	0.00	0.00	2.45	6.00a	0.00	0.00
Control+LPS	±0.00	±0.00	±0.00	±0.00	±2.75	±4.11a	±0.00	±0.00

^aP<0.05 vs control group;

^cP<0.05 vs AP group.