Pituitary adenylate cyclase activating-peptide and its receptor antagonists in development of acute pancreatitis in rats

doi:10.3748/wjg.v11.i4.538

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 28, 2005; 11(4): 538-544
Published online Jan 28, 2005. doi: 10.3748/wjg.v11.i4.538

Table 1 Ascites/peritoneal fluid and their gross appearance (action of PACAP) (mean±SE).

Group	Volume ofascites (mL)	Ascites amylase(IU/L)	Nucleated cells in ascites (×10⁸/L)	Saponifi -cation (n)	Hemorr -hage (n)
Control		213±44/7	1.71±1.02/9
Caerulein-induced pancreatitis		39075±50341/3	7.54±2.85/4^a
Sodium taurocholate-induced pancreatitis	3.55±1.86/12	11 463±5 486/10^a	2.9±3.06/8	2	3
PACAP 5 μg/kg
PACAP 10 μg/kg
PACAP 15 μg/kg
PACAP 30 μg/kg			3.26±1.89/6
Sodium taurocholate+PACAP 5 μg	2.33±0.82/6	26917±11145/6^a	2.3±0.98/6	2/6	4/6
Sodium taurocholate+PACAP 10 μg	4.77±2.44	5956±3546/8^a	1.76±1.02/11	7	11
Caerulein+PACAP 15 μg	0.55±0.96	926/1		1	1
Caerulein+PACAP 30 μg	0.91±0.83	63918±82884/8	1.73±1.23/8	1	3

^aP<0.05 vs normal control.

Table 2 Histological scoring (action of PACAP) (mean±SE).

Group	Hemorrhage	Parenchymal necrosis	Vacuolization	Inflammation
Caerulein-induced pancreatitis			1.67±0.60	2.5±0.22
Sodium taurocholate-induced pancreatitis	1.5±0.67	2.5±0.49	0.5±0.49	3
PACAP 5 μg			0.33±0.33	0.17±0.17
PACAP 10 μg		0.17±0.17	0.67±0.49	1.33±0.61
PACAP 15 μg			0.33±0.33	1.5±0.67
PACAP 30 μg		0.17±0.17	0.83±0.54	1.5±0.5
Sodium taurocholate+PACAP 5 μg	1.67±0.49	2.67±0.33	2±0.63	3
Sodium taurocholate +PACAP 10 μg	2±0.63	2.5±0.5	1.5±0.56	3
Caerulein+PACAP 15 μg	1.5±0.5	2±0.63	2.5±0.22	1.67±0.60
Caerulein+PACAP 30 μg	1.83±0.60	2.17±0.31	2.33±0.49	2.17±0.48

Table 3 Dry weight/wet weight ratio of pancreas and serum amylase (action of PACAP) (mean±SE).

Group	Pancreatic dry weight/wet weight ratio (%)	Serum amylase (IU/L)
Normal control	29.21±5.657	520.8±163.27
Caerulein-induced pancreatitis	21.83±3.013^a	3699.33±3826.56^a
Sodium taurocholate-induced pancreatitis	17.52±1.505^a	3690.87±2277.99^a
PACAP 5 μg	25.86±1.974	818.09±404.76^a
PACAP 10 μg	24.81±1.312	675.55±271.44
PACAP 15 μg	23.88±2.532	671.36±151.98^a
PACAP 30 μg	25.17±0.897	899.09±474.49
Sodium taurocholate+PACAP 5 μg	19.18±2.102^a	2944.33±1182.47^a
Sodium taurocholate+PACAP 10 μg	20.87±5.597^a	1986.91±710.97^a
Caerulein+PACAP 15 μg	17.66±4.652^a	4053.55±2164.07^a
Caerulein+PACAP 30 μg	13.45±2.045^a	5243.46±3769.73^a

^aP<0.05 vs normal control.

Table 4 ELISA for PACAP in pancreas and duodenum (mean±SE).

Group	Relative concentrations
Group	In pancreas	In duodenum
Normal control	0.02215±0.01123 (n = 4)	0.000335±0.000074 (n = 5)
Caerulein-induced AP	12.0712±8.40536 (n = 4)^a	0.003797±0.002301 (n = 5)^a
Sodium taurocholate-induced AP	0.2799±0.22932 (n = 4)^a	0.001564±0.000529 (n = 4)^a

^aP<0.05 vs normal control.

Table 5 Ascites and its gross appearance (effect of PACAP receptor antagonists) (mean±SE).

Group	Volume of ascites (mL)	Ascites amylase(IU/L)	Nucleated cell countof ascites (×10⁸/L)	Saponi-fication (n)	Hemorr-hage (n)
Normal control		213±44/7	1.71±1.02/9
Caerulein-induced pancreatitis		39075±50341/3	7.54±2.85/4^a
Sodium taurocholate-induced pancreatitis	3.55±1.86/12	11463±5486/10^a	2.9±3.06/8	2/12	3/12
10 μg PACAP6-27		2000±1273/2	3.43±1.22/6^a
100 μg PACAP6-27		600±283/2	1.39±0.98/2
Caerulein+10 μg PACAP6-27		5725±3104/4^a	7.04±4.93/5^a
Caerulein +100 μg PACAP6-27	3/1	90250±94964/2^a	1.53±0.82/2
Sodium taurocholate+10 μg PACAP6-27	2.58±0.86/6	49467±45529/6^a	2.35±1.81/6	1/6	3/6
Sodium taurocholate+100 μg PACAP6-27	3.42±1.99/6	64083±40650/6^a	3.44±1.7/6^a	4/6	2/6
10 μg (4-Cl-D-Phe6, Leu17) VIP		750±495/2^a	5.52±2.38/4^a
100 μg (4-Cl-D-Phe6, Leu17) VIP		201	3.4/1
Caerulein+10 μg (4-Cl-D-Phe6, Leu17) VIP		6100±1697/2^a	5.38±2.17/5^a
Caerulein+100 μg (4-Cl-D-Phe6, Leu17) VIP		217	1.06±0.51/3
Sodium taurocholate+10 μg (4-Cl-D-Phe6, Leu17) VIP	2.25±0.88/6	32700±13611/6^a	2.25±0.86/6		3/6
Sodium taurocholate+100 μg (4-Cl-D-Phe6, Leu17) VIP	4.17±1.13/6	68817±46943/6^a	2.51±1.5/4		2/6

^aP<0.05 vs normal control.

Table 6 Pancreatic dry weight/wet weight ratio and serum amylase (effect of PACAP receptor antagonists) (mean±SE).

Group	Dry weight/wet weight ratio (%)	Serum amylase (IU/L)
Normal control	29.21±5.657	520.8±163.27
Caerulein-induced pancreatitis	21.83±3.013^a	3699.33±3826.56^a
Sodium taurocholate-induced pancreatitis	17.52±1.505^a	3690.87±2277.99^a
10 μg PACAP6-27	25.25±2.286^a	1464.33±265.6^a
100 μg PACAP6-27	26.21±2.577	1692.17±312.18^a
Caerulein+10 μg PACAP6-27	22.75±3.523^a	2484.33±1459.64^a
Caerulein+100 μg PACAP6-27	21.4±4.152^a	6485.5±3352.84^a
Sodium taurocholate+10 μg PACAP6-27	20.81±3.94^a	5026.83±3697.35^a
Sodium taurocholate+100 μg PACAP6-27	17.99±3.594^a	7667.67±4270.93^a
10 μg (4-Cl-D-Phe6, Leu17) VIP	25.61±2.389^a	1 337.17±314.02^a
100 μg (4-Cl-D-Phe6, Leu17) VIP	26.77±1.377	1781±527.65^a
Caerulein+10 μg (4-Cl-D-Phe6, Leu17) VIP	24.33±0.939^a	2875.33±582.98^a
Caerulein+100 μg (4-Cl-D-Phe6, Leu17) VIP	22.36±2.13^a	8307.83±2003.41^a
Sodium taurocholate+10 μg (4-Cl-D-Phe6, Leu17) VIP	16.89±1.18^a	4684.33±993.55^a
Sodium taurocholate+100 μg (4-Cl-D-Phe6, Leu17) VIP	18.71±4.048^a	7264.67±2834.91^a

^aP<0.05 vs normal control.

Table 7 Histological scoring (effect of PACAP receptor antagonists) (mean±SE).

Group	Hemorrhage	Parenchymal necrosis	Vacuolization	Inflammatory infiltration
Caerulein-induced pancreatitis			1.67±0.60	2.5±0.22
Sodium taurocholate-induced pancreatitis	1.5±0.67	2.5±0.49	0.5±0.49	3
10 μg PACAP6-27				1.5±0.5
100 μg PACAP6-27				0.83±0.54
Caerulein+10 μg PACAP6-27	0.83±0.54	0.17±0.17	1.83±0.60	2.5±0.5
Caerulein+100 μg PACAP6-27	1.67±0.49	1±0.52	2.17±0.31	2.5±0.22
Sodium taurocholate+10 μg PACAP6-27	1.5±0.67	3	1.33±0.61	3
Sodium taurocholate+100 μg PACAP6-27	1.5±0.5	2.83±0.17	2±0.52	3
10 μg (4-Cl-D-Phe6, Leu17) VIP			0.33±0.33	2±0.63
100 μg (4-Cl-D-Phe6, Leu17) VIP			1±0.63	1.67±0.60
Caerulein+10 μg (4-Cl-D-Phe6, Leu17) VIP	0.17±0.17	0.17±0.17	0.67±0.40	2±0.51
Caerulein+100 μg (4-Cl-D-Phe6, Leu17) VIP	0.33±0.33	1.67±0.49	2.17±0.48
Sodium taurocholate+10 μg (4-Cl-D-Phe6, Leu17) VIP	1.5±0.56	2.5±0.49	1.17±0.60	3
Sodium taurocholate+100 μg (4-Cl-D-Phe6, Leu17) VIP	1.5±0.67	2.33±0.33	1.83±0.60	3

Table 8 Functional capillary densities of pancreas (mean±SE).

Group	FCD (cm^-1)	Calibrated FCD (cm^-1)
Normal control	22.93±5.189
Caerulein-induced pancreatitis	25.71±3.398^a	34.4
Sodium taurocholate-induced pancreatitis	21.9±5.681	36.51
PACAP 5 μg	21.68±4.268	24.49
PACAP 10 μg	19.52±3.837^a	22.98
PACAP 15 μg	19.6±4.949^a	23.97
PACAP 30 μg	18.56±6.021^a	21.53
Sodium taurocholate+PACAP 10 μg	18.71±3.383^a	26.19
Caerulein+PACAP15 μg	22.68±3.62	37.51
Caerulein+PACAP 30 μg	18.28±3.772^a	39.7

^aP<0.05 vs normal control.

Citation: Chen YD, Zhou ZG, Wang Z, Gao HK, Yan WW, Wang C, Zhao GP, Peng XH. Pituitary adenylate cyclase activating-peptide and its receptor antagonists in development of acute pancreatitis in rats. World J Gastroenterol 2005; 11(4): 538-544
URL: https://www.wjgnet.com/1007-9327/full/v11/i4/538.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i4.538