Brief Reports
Copyright ©The Author(s) 2005.
World J Gastroenterol. Oct 21, 2005; 11(39): 6202-6207
Published online Oct 21, 2005. doi: 10.3748/wjg.v11.i39.6202
Table 1 Sequences of primers and probes
Target gene(amplicon size)Sequence (5'-3')
HAI-1Upstream primerTGA GGA AGA GCA GCA GTG CC
(151 bp)Downstream primerGGC TAC CAC CAC CAC AAT GC
ProbeFAM -CCA GCA CAG GCT CTG TGG AGA
TGG C -TAMRA
SNC19/Upstream primerGGG ACA CAC CCA GTA TGG AGG
matriptase
(168 bp)Downstream primerCCG GAA TCA CCC TGG CAG GA
ProbeFAM -TCC TGC AAA AGG GTG AGA TCC
GCG -TAMRA
GAPDHUpstream primerCTT AGC ACC CCT GGC CAA G
(151 bp)Downstream primerGAT GTT CTG GAG AGC CCC G
ProbeFAM -CAT GCC ATC ACT GCC ACC CAG
AAG A -TAMRA
Table 2 Expression of HAI-1 and SNC19/matriptase in normal and malignant tissues of gastrointestinal tract [M(QR)]
Gastric tissues
Colorectal tissues
HAI-1SNC19HAI-1:SNC19HAI-1SNC19HAI-1:SNC19
Normal tissues0.012(0.021)0.010(0.014)1.420(1.662)0.014(0.022)0.010(0.014)0.971(1.415)
Malignant tissues0.005(0.013)0.004(0.005)1.365(1.820)0.009(0.007)0.005(0.005)1.520(1.215)
Z-3.28-4.651-0.121-3.1-2.731-0.999
P0.00100.9040.0020.0060.318
Table 3 Correlation between HAI-1 and SNC19/matriptase expression and clinicopathology in gastric cancer tissues [M(QR)]
PatientsHAI-1SNC19HAI-1:SNC19
Age (yr)
<40 yr30.005 (0.011)0.003 (0.002)1.415 (1.416)
40-60 yr170.005 (0.016)0.005 (0.006)1.680 (2.022)
>60 yr200.005 (0.001)0.003 (0.003)0.882 (0.144)
Gender
Men280.005 (0.013)0.004 (0.004)1.220 (1.631)
Women120.005 (0.020)0.003 (0.005)1.680 (1.571)
Location
Cardia70.005 (0.004)0.003 (0.002)1.430 (0.210)
Body70.007 (0.023)0.004 (0.005)2.620 (2.846)
Pylorus170.005 (0.015)0.005 (0.005)1.080 (1.631)
Unkown90.005 (0.110)0.003 (0.004)1.220 (1.219)
Tumor histology
adenocarcinoma180.005 (0.008)0.004 (0.004)1.365 (1.442)
Mucoid carcinoma70.005 (0.021)0.003 (0.008)1.740 (1.626)
Poorly differentiated carcinoma110.003 (0.016)0.003 (0.004)0.882 (2.377)
Unkown40.005 (0.114)0.005 (0.004)1.555 (1.386)
N factor
N0120.017 (0.032) a0.004 (0.005)2.280 (3.148) e
N1/N2280.004 (0.005)0.004 (0.005)1.080 (1.489)
UICC stage
I/II100.017 (0.020) c0.004 (0.005)2.280 (2.718) g
III/IV300.004 (0.007)0.003 (0.004)1.245 (1.541)
Histological grade
Well30.011 (0.008)0.003 (0.002)2.870 (1.753)
Moderately150.005 (0.004)0.004 (0.004)1.400 (1.121)
Poorly220.004 (0.017)0.003 (0.007)1.150 (0.470)
Table 4 Correlation between HAI-1 and SNC19/matriptase expression and clinicopathology in colorectal cancer tissues [M(QR)]
PatientsHAI-1SNC19HAI-1:SNC19
Age yr
<40 yr30.004 (0.003)0.005 (0.002)0.778 (0.349)
40-60 yr100.007 (0.102)0.003 (0.003)0.798 (1.096)
>60 yr230.009 (0.104)0.005 (0.005)0.677 (0.459)
Unkown1
Gender
Men130.005 (0.109)0.005 (0.006)0.944 (0.728)
Women240.009 (0.100)0.005 (0.004)0.667 (0.592)
Location
Cecum/ascending colon90.009 (0.101)0.005 (0.006)0.660 (0.482)
Transverse colon20.003 (0.003)0.002 (0.002)0.862 (0.377)
Descending/sigmoid colon60.010 (0.004)0.006 (0.002)0.663 (0.715)
Rectum190.005 (0.009)0.004 (0.005)0.778 (1.158)
Unknown1
Tumor histology
Adenocarcinoma320.005 (0.009)0.004 (0.005)0.734 (0.788)
Mucoid carcinoma40.009 (0.012)0.005 (0.004)0.511 (0.194)
Poorly differentiated carcinoma1
N factor
N0180.010(0.015)0.005(0.009)1.470(0.699)
N1/N2190.007(0.005)0.005(0.004)1.480(0.980)
Dukes’ stage
A70.011(0.015)0.004(0.008)1.790(3.860)
B100.010(0.016)0.006(0.015)1.290(1.243 )
C/D200.006(0.006)0.005(0.005)1.385(0.955)
Histological grade
Well80.005(0.010)0.006(0.024)0.891(1.482)
Moderately240.009(0.009)0.004(0.005)1.485(1.250 )
Poorly50.009(0.012)0.005(0.005)1.790(1.000)