p53 negativity, CDC25B positivity, and metallothionein negativity are predictors of a response of esophageal squamous cell carcinoma to chemoradiotherapy

Table 1 Characteristics of patients (mean±SD, n)

Parameters	Values
Sex (male/female)	Mar-33
Age (range)	63.3±9.2 (42-78 yr)
Histopathology
Well-differentiated	6
Moderately differentiated	23
Poorly differentiated	7
Stage (UICC)
I	4
II	7
III	18
IVa	7

Table 2 Relation between immunohistochemical expressions of biological markers and response of esophageal squamous cell car-cinoma to CRT (n)

Markers	Responder	Non-responder	P
	(CR+PR)	(NC+PD)
p53(-)	14	1	0.0095
p53(+)	11	10
MT (-)	21	5	0.019
MT (+)	4	6
CDC25B (-)	7	8	0.0129
CDC25B (+)	18	3

Spearman’s rank correlation test. z

Table 3 Relation between expressions of biological markers and response of esophageal squamous cell carcinoma to CRT (n)

Markers	Responder	Non-responder	P
	(CR+PR)	(NC+PD)
Bax (-)	17	8	0.799
Bax (+)	8	3	0.799
Bcl-2 (-)	18	8	0.836
Bcl-2 (+)	7	3	0.836
Cyclin D1 (-)	15	6	0.305
Cyclin D1 (+)	10	5	0.305
VEGF (-)	10	5	0.936
VEGF (+)	15	6	0.936
Ki67 (-)	4	1	0.586
Ki67 (+)	21	10	0.586

Spearman’s rank correlation test.

Table 4 Relation between effects of CRT and immunohistochemical staining

	Immunoreactivity/response				P
p53	-	-	+	+	0.027
CDC25B	+	-	+	-
Responder	9	5	9	2
Non-responder	0	1	3	7
MT	-	-	+	+
0.0305
CDC25B	+	-	+	-
Responder	16	5	2	2
Non-responder	2	3	1	5

χ² test.