DNA ploidy analysis and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma

Table 1 Relationship between expression of MMP-9, TIMP-2 or E-cadherin, and histopathological features in gastric carcinoma

	n	Positive		TIMP-2	Rate (%)		P
		MMP-9	P		P	E-cadherin
Differentiation
Well	16	50	>0.05	62.5	>0.05	75	<0.01
Moderate	18	66.7		55.6		61.1
Poor	36	69.4		38.9		30.6
Signet ring cell type	29	72.4		31		27.6
Tumor recurrence
Primary carcinoma	91	65.9	>0.05	44	>0.05	44	>0.05
Recurrent carcinoma	8	75		37.5		25
Carcinoma and non-neoplastic
Gastric carcinoma	99	66.7	<0.01	43.4	>0.05	42.4	<0.001
Non-neoplastic gastric mucosa	16	31.3		31.3		87.5
Lauren’s classification
Intestinal type	67	58.2a	<0.05	46.3	>0.05	53.7a	<0.05
Diffuse type	22	90.9		36.4		13.6
Mixed type	10	70		40		30
Borrmann’s classification
Types I–II	9	33.3c	<0.05	66.7	<0.05	55.6c	<0.05
Type III	70	65.7		47.1		42.9
Type IV	20	85		20		25

^aP<0.05 vs diffuse type;

^cP<0.05 vs type IV.

Table 2 Relationships between expression of MMP-9, TIMP-2 or E-cadherin, and clinical features in gastric carcinoma

	n	Positive		TIMP-2	Rate (%)		P
		MMP-9	P		P	E-cadherin
Tumor size
5 cm	53	66.0	>0.05	41.5	>0.05	39.6	>0.05
<5 cm	46	67.4		45.7		45.7
LN metastasis
Negative	46	54.3	<0.01	56.5	<0.05	54.3	<0.05
Positive	53	79.2		32.1		32.1
Metastasis
Primary carcinoma	99	66.7	<0.05	43.4	>0.05	42.4	>0.05
Metastatic LN	25	92.0		36.0		20.0
Metastatic carcinoma	16	81.7		43.8		37.5
Depth of invasion
Submucosa	7	28.6c	<0.05	71.4c	<0.05	85.7c	<0.05
Superficial muscular layer	27	59.3		59.3		48.1
Deep muscular layer or serosa	65	73.8		33.8		35.4
TNM stage
Stage II	8	37.5e	<0.05	50.0	>0.05	62.5	>0.05
Stage III	16	50.0		43.8		50.0
Stage IV	75	73.3		42.7		38.7

^cP<0.05 vs deep muscular layer or serosa;

^eP<0.05 vs stage IV.

Table 3 Correlations between MMP-9 and TIMP-2 expression

	TIMP-2
MMP-9	-	+
+	32	34
-	24	9

Table 4 Correlations between E-cadherin and MMP-9 expression

MMP-9	E-cadherin
	-	+
+	46	20
-	11	22

Table 5 Correlations between TIMP-2 and E-cadherin expression

E -cadherin	TIMP-2
	-	+
+	22	20
-	35	22

Table 6 Correlation between DNA ploidy or SPF and histopathological or clinical features in gastric carcinoma

	n	Aneuploid rate (%)	P	n (SPF>15%)	P
Tumor size
5 cm	29	65.5	>0.05	23	>0.05
<5 cm	18	55.6		9
Differentiation
Well or moderate	19	42.1	<0.05	9	<0.05
Poor or signet ring cell type	28	75		23
Depth of invasion
Submucosa	7	28.6	>0.05	3	>0.05
Muscular layer or serosa	40	67.5		29
LN metastasis
Negative	30	80	<0.001	24	<0.05
Positive	17	29.4		8
Metastasis
Primary carcinoma	47	61.7	>0.05	32	>0.05
Metastatic carcinoma	4	100		3
Carcinoma and noncarcinoma
Gastric carcinoma	47	61.7	<0.05	32	<0.05
Noncarcinoma gastric mucosa	6	16.7		1