Immunohistochemical study on gastrointestinal endocrine cells of four reptiles

Table 1 Details of antisera and main reagents used

Antisera and reagent	Donor	Code number	Working dilution	Source
Human 5-HT	Rabbit	ZA-0231	1:100	ZYMED Lab. Inc., USA
Human GAS	Rabbit	ZA-0115	1:50	Same as above
Human SS	Rabbit	ZA-0232	1:50	Same as above
Human PP	Rabbit	ZA-0211	1:50	Same as above
Human SP	Rabbit	ZA-0235	Working solution	Same as above
Human GLU	Rabbit	ZA-0119	1:100	Same as above
Human INS	Mouse	ZM-0155	1:50	Same as above
S-P Kit		SP-9001	Working solution	Zhongshan Biotech Co. LTD., Beijing, China
S-P Kit		SP-9002	Working solution	Same as above
DAB		ZLI-9030	0.6 g/L	Same as above

SP-9002 was only used in detecting INS-IR cells.

Table 2 Distribution and density of endocrine cells in GIT of G. japonicus (cells/400× field, mean±SD)

	5-HT	SS	GAS	GLU	SP	PP	INS
Cardia	4.8±0.8	5.5±1.9	0	4.3±1.2	3.6±1.1	0	0
Fundus	5.6±1.4	6.0±1.2	0	11.7±0.7	14.6±1.8	0	0
Pylorus	4.1±1.5	9.6±1.0	0	16.3±2.2	11.2±2.2	0	0
Duodenum	8.6±2.2	1.0±0.5	3.8±0.8	0	0	0	0
Jejunum	7.0±2.9	0	1.4±0.5	0	0	0	0
Ileum	7.8±1.2	0	0	0	0	0	0
Rectum	7.0±1.6	0	0	0	0	0	0

Table 5 Distribution and density of endocrine cells in GIT of E. elegans (cells/400× field, mean±SD)

	5-HT	SS	GAS	GLU	SP	PP	INS
Cardia	1.6±1.1	1.1±1.2	0	0	0	0	0
Fundus	4.2±1.0	2.3±0.7	0	0	0	0	0
Pylorus	9.1±1.7	4.6±1.9	21.4±11.6	0	0	0	0
Duodenum	3.1±0.9	0	1.3±1.1	0	0	0	0
Jejunum	1.4±1.4	0	0	0	0	0	0
Ileum	3.4±1.6	0	0	0	0	0	0
Rectum	5.2±2.0	0	0	0	0	0	0