Novel genetic variations of the p53R2 gene in patients with colorectal adenoma and controls

Table 1 Primers for PCR amplification, sequencing and RFLP (GenBank accession no. AC021004)

Number	Name	Sequence	PCR product (bp)
1	Exon 1 (F)	5’-GGAGAGTCACTCAATGGAC-3’	302
2	Exon 1 (R)	5’-CCTGCAACTTGCAATCTAAC-3’
3	Exon 2 (F)	5’-GGAACACTGCACTATAGGATG -3’	291
4	Exon 2 (R)	5’-CCCTGACTTCCTTAGATG-3’
5	Exon 3 (F)	5’-GCTAAAGGAGAACAAAAG-3’	218
6	Exon 3 (R)	5’-CCAAACACTTTGAGAAGAAC-3’
7	Exon 4 (F)	5’-GCTCAGAAACTTCCATACTTG-3’	336
8	Exon 4 (R)	5’-CGATAATGCTGATGTCCAG-3’
9	Exon 5 (F)	5’-CGTACTGGATAGTCACAC -3’	287
10	Exon 5 (R)	5’-CGAGATCGTGCCACTGCAC-3’
11	Exon 6 (F)	5’-GGCACTAACTTGTGTATTTTG-3’	284
12	Exon 6 (R)	5’-CTAGAAAAACATTCCATTCC-3’
13	Exon 7 (F)	5’-GGTACAAACATCAGAGAAAG-3’	224
14	Exon 7 (R)	5’-CCATCACCATATGCAAATAG-3’
15	Exon 8 (F)	5’-GGTCCTTGCTTTCCTATA-3’	239
16	Exon 8 (R)	5’-CGAAAGTCCTCTTTCTA-3’
17	Exon 9 (F)	5’-GCAGAGGAAAATAGACTAC-3’	317
18	Exon 9 (R)	5’-GGTTTTGAGAAACCTGAC-3’
19	Intron 1 (F)	5’-CAGGACTCAGTAGAGGAGCT-3’	550
20	Intron 1 (R)	5’-CACAGGGTCAGTGACACTG-3’

Table 2 Allele distributions at four polymorphic loci in the human p53R2 gene from a case-control study of incident sporadic colorectal adenoma

	Cases (n = 163)		Controls (n = 210)		P
	n	%	n	%
DdeI
CC	143	87.7	185	85.6
GG	1	0.6	2	0.9
CG	19	11.7	29	13.4	0.82
NdeI
GG	131	80.4	178	82.4
AA	1	0.6	6	2.8
AG	30	18.4	31	14.4	0.25
HhaI
TT	159	97.6	210	97.2
CT	4	2.4	6	2.8
CC	0	0	0	0	0.9
Insertion
(+)	4	2.4	0	0	-