Impact of antithrombin III on hepatic and intestinal microcirculation in experimental liver cirrhosis and bowel inflammation: An in vivo analysis

Table 1 Cardiorespiratory parameters at the beginning of intravital videomicroscopy (mean±SD)

	Cirrhosis	Cirrhosis and IBD	ATIII treatment group(cirrhosis and IBD)	Controls
Mean arterial blood	107±8.4	102±8.7	106±7.8	112±7.3
pressure (mmHg)
Heart rate (bpm)	310±9.2	318±7.4	312±8.2	304±8.4
paO2 (mmHg)	89.1±5.3	86.4±4.3	88.6±5.4	92.3±4.8

Table 4 Data of blood samples taken at the end of experiments (mean±SD)

	Cirrhosis	Cirrhosis and IBD	ATIII treatment group (cirrhosis and IBD)	Controls
ASOT (U/L)	63.67±10.42a	109.5±39.18c	29.17±7.62e	28.67±4.85
ALT (U/L)	31.83±4.84a	84.67±32.95c	21.17±13.61e	16.67±1.11
GGT (U/L)	5.33±1.49	14.83±3.48c	7.67±1.25e	3.17±0.69
AP (U/L)	130.5±35.75a	268.83±53.25c	69.83±12.21e	56.83±2.67
Prothrombin	75.3±5.4a	58.7±4.3c	74.6±4.9e	102.4±2.7
Time (%)
ATIII (IU)	108.5±14.59	71.67±8.94c	138.50±2.29e	125.0±8.96

^aP<0.05 cirrhosis vs controls;

^cP<0.05 cirrhosis+IBD vs cirrhosis;

^eP<0.05 ATIII vs cirrhosis+IBD.

Table 2 Microcirculatory parameters of the liver, mesentery and ileal mucosa (mean±SD)

	Cirrhosis	Cirrhosis and IBD	ATIII treatment group (cirrhosis and IBD)	Controls
Vessel diameter (µm)
Liver	27.86±2.03a	28.15±1.72	28.08±1.27	23.03±0.62
Mesentery	32.06±8.91	33.51±5.16	42.51±7.04e	33.46±11.66
Mucosa	7.51± 0.30	6.32±0.41	7.21±0.52	7.20±0.23
RBC velocity (mm/s)
Liver	0.93±0.09a	1.08±0.13c	1.16±0.11	1.22±0.18
Mesentery	2.81±0.49	3.28±0.42	2.93±0.41	3.11±0.35
Mucosa	2.03±0.15	1.81±0.13c	2.11±0.12	2.18±0.16
Volumetric blood flow (nL/min)
Liver	32.1±0.43	39.5±0.43c	40.9±0.62	31.2±0.6
Mesentery	135.1±3.56a	149.8±2.41c	243.1±3.71e	156.5±4.3
Mucosa	5.3±0.31	3.49±0.6c	5.13±0.4e	5.4±0.25

^aP<0.05 cirrhosis vs controls;

^cP<0.05 cirrhosis+IBD vs cirrhosis;

^eP<0.05 ATIII vs cirrhosis+IBD.

Table 3 Results of intravital videomicroscopy: LEI in the liver and mesentery (mean±SD)

	Cirrhosis	Cirrhosis and IBD	ATIII treatment group (cirrhosis and IBD)	Controls
Adherent leukocytes (n/100 µm)
Liver	1.91±0.28a	5.40±1.65c	3.97±1.04e	0.5±0.5
Mesentery	2.54±1.19	5.24±1.23c	4.36±1.19	1.62±0.85
Rolling leukocytes (n/100 µm)
Liver	4.80±0.90a	5.32±1.29	3.40±0.54e	2.33±0.75
Mesentery	7.68±3.18	10.34±8.94	10.82±7.29	6.88±1.94

^aP<0.05 cirrhosis vs controls;

^cP<0.05 cirrhosis+IBD vs cirrhosis;

^eP<0.05 ATIII vs cirrhosis+IBD.