Microsatellite instability in gastric cancer and pre-cancerous lesions

Table 1 Sequences of primers for PCR analysis

Repeat sequence	Primers	Amplicated fragment(bp)
BAT-25	5’-TCGCCTCCAAGAATGTAAGT-3’	-90
	5’-TCTGCATTTTAACTATGGCTC-3’
BAT-26	5’-TGACTACTTTTGACTTCAGCC-3’	80-100
	5’-AACCATTCAACATTTTTAACCC-3’
D5S346	5’-ACTCACTCTAGTGATAAATCG-3’	96-122
	5’-AGCAGATAAGACAGTATTACTAGTT-3’
D17S250	5’-GGAAGAATCAAATAGACAA-3’	-150
	5’-GCTGGCCATATATATATTTAAACC-3’
D2S123	5’-AAACAGGATGCCTGCCTTTA-3’	197-227
	5’-GGACTTTCCACCTATGGGAC-3’

Table 2 Characteristics of 36 gastric cancer patients

Characteristic	MSS (n = 15)	MSI-L (n = 14)	MSI-H (n = 7)
Average age	58	62	58
Sex
Male	10	11	4
Female	5	3	3
Differentiation grade
well-moderate	7	9	6
poor	8	5	1
Tumor location
Distal	8	8	5
Proximal	7	6	2
Lymph node spread
Absent	9	7	4
Present	6	8	3

Table 3 Characteristics of 41 patients with dysplasia

Characteristic	MSS (n = 30)	MSI-L (n = 8)	MSI-H (n = 3)
Histological grade
Mild	16	3	1
Moderate	13	2	1
Poor	1	3	1

Table 4 Characteristics of 51 patients with intestinal metaplasia

Characteristic	MSS (n = 42)	MSI-L (n = 8)	MSI-H (n = 1)
Sex
Male	25	2	0
Female	17	6	1
Histological grade
Mild	28	0	0
Moderate	13	7	0
Poor	1	0	1