Lewis blood genotypes of peptic ulcer and gastric cancer patients in Taiwan

Table 1 Primer sequences and restriction enzymes of mutation in Se and Le genes

Mutation	Primer sequence	Enzyme
Se gene
A385T	UP1: GATGGAGGAGGAATACCGCCTC	Ear I
	DP2: GATCTCCTGGCGGAGGTGGTGGTAGAAGATC
G428A	Identical to A358T primer pairs	Bgl II
C571T	UP: AGGAGATCCTCCAGGAGTTCA	Dde I
	DP: AGAAGGAGAAAAGGTCTCAAAGG
G849A	Identical to C571T primer pairs	Dde I
C628T	UP: AGTGTGGAAGGGGGTGGTGCCC	Bgl I
	DP: CCACTCTGGCAGGAAGGC
Fusion gene	UP: CTGCCTCCTGACCATGTCC	Pst I
	DP: identical to reverse primer of C628T
Le genes
T202C and C314T	UP: CCACCCTCCTGATCCTGCTC	Msp I (T202C)
	DP: GATATCCCAGTGGTGCACGATGATGATC	Bcl I (C314T)
C445A	UP: identical to UP of T202 C and C314 T	BstN I
	DP: GAGATTGAAGTATCTGTCCAAGGC
G508A	UP: TCAACTTGGAGCCACACCCT	Alu I
	DP: AGTTGGACACCGCCCAGGCCACCAG
A1007C	UP: GCTCCTTCCGCTGGGCACTAG	Alu I
	DP: TGGCCACAAAGGACTCCAGC
T1067A	UP: GTACCAGACGGTGCGATGCA	Nsi I
	DP: identical to DP of A1007 C

¹UP: upstream primer; ²DP: downstream primer; The mutated base is underlined.

Table 2 Correlation between Lewis phenotype and H pylori infection in peptic ulcer disease

Phenotypes	Lea-b+ (n = 230)	Lea-b- (n = 59)	Lea+b- (n = 14)	Lea+b+ (n = 44)	P
H pylori status					0.5662
Positive	195	54	12	39
Negative	35	5	2	5
Prevalence %	84.7	91.5	85.7	88.5

Table 3 Correlation between Lewis genotype and H pylori infection in peptic ulcer disease

Genotypes	SeLe/SeLe (n = 33)	SeLe/seLe (n = 65)	SeLe/Sele (n = 42)	SeLe/Sele (n = 90)	SeLe/Sele (n = 3)	SeLe/Sele (n = 19)	SeLe/Sele (n = 37)	SeLe/Sele (n = 46)	SeLe/Sele (n = 12)	P
H pylori status										0.446
Positive	28	59	36	72	2	17	33	41	12
Negative	5	6	6	18	1	2	4	5	0
Prevalence %	84.8	90.7	85.7	80	66.6	89.4	89.1	89.1	100

Table 4 Lewis genotypes in peptic ulcer and gastric cancer

Genotypes	Peptic ulcer n = 347	Gastric cancer n = 51	Normal n = 101
SeLe/SeLe	33	2	7
SeLe/seLe	65	11	25
SeLe/Sele	42	4	10
SeLe/sele	90	15	21
Sele/Sele	3	3	5
Sele/sele	19	4	7
seLe/seLe	37	3	12
seLe/sele	46	5	9
sele/sele	12	4	5
P	0.1291	0.8812

¹P = 0.129 peptic ulcer vs normal controls; ²P = 0.881 gastric cancer vs normal controls.

Table 5 ABO blood types and susceptibility to H pylori infection (n = 230)

Blood types	A (n = 44)	B (n = 54)	O (n = 122)	AB (n = 10)	P
H pylori status					0.255
Positive	34	49	106	8
Negative	10	5	16	2
Prevalence %	77.2	90.7	86.8	80