Nitrative and oxidative DNA damage in intrahepatic cholangiocarcinoma patients in relation to tumor invasion

Table 1 Relationship of DNA damage with lymphatic and neural invasion in ICC patients

Immuno-histologicalgrading	8-oxodGNumber ofpatients (%)		8-NitroguanineNumber ofpatients (%)		HIF-1αNumber ofpatients (%)	Number (%)of patientswithlymphaticinvasion (8-oxodG)	Number (%)of patientswithlymphaticinvasion (8-nitroguanine)	Number (%) ofpatients withneuralinvasion(8-oxodG)	Number (%)of patientswithneuralinvasion (8-nitroguanine)
	Cancerous	Non-cancer	Cancerous	Non-cancer	Cancerous	Cancerous	Cancerous	Non-cancer	Non-cancer
ICC (37)
0	-	5 (13.51)	11 (29.73)	17 (46)	-	-	9/11 (81.82)	1/5 (20)	8/17 (47.1)
+	11 (29.73)	17 (45.94)	13 (35.14)	19 (51.4)	10 (27.03)	6/11 (54.55)	10/13 (76.92)	14/17 (82.4)	16/19 (84.2)
++	11 (29.73)	10 (27.03)	8 (21.62)	1 (2.70)	18 (48.65)	10/11 (90.91)	6/8 (75)	5/10 (50)	0/1 (0)
+++	15 (40.54)	5 (13.51)	5 (13.51)	-	9 (24.32)	14/15 (93.33)	5/5 (100)	4/5 (80)
P-value	P = 0.004		P = 0.007			P = 0.018	P = 0.679	P = 0.042	P = 0.026
Healthy (9)
0		2 (22.22)		7 (77.77)
+		7 (77.77)		2 (22.2)

Non-cancer = noncancerous tissues. 0 = no positive cell, + = positive in few areas or cells, ++ = moderately positive and +++ = predominately positive. ICC samples, comprising 37 matched cancerous and adjacent noncancerous tissues and 9 healthy subjects who died from accidental cases were collected at the time of surgery. ICC patients included 26 men and 11 women with the mean age 53±11 years. These subjects were verified by histopathological study and comprised well- (15 patients, including 2 patients of papillary), moderately- (11 patients) and poorly (11 patients) differentiated adenocarcinoma. The International Union Against Cancer TNM classification and staging system were used for tumor assessment.