Mutation of DNA polymerase β in esophageal carcinoma of different regions

Table 1 Mutation analysis of polβ in high-incidence areas

Specimen	Gene mutation
	Carcinoma	Corresponding adjacent normal tissue
H1	-	-
H2	-	-
H3	-	-
H4	660 nt A→G	-
H5	462 nt G→T,	-
	177-234 nt deletion
H6	-	-
H7	64 nt G→C, 665 nt T→C	-
H8	462 nt G→T, 660 nt A→G,	660 nt A→G
	177-234 nt deletion
H9	-	-
H10	177-234 nt deletion,	-
	454 nt T→C 466 nt G→A
H11	-	-
H12	375 nt A→G	-
	177-234 nt deletion
H13	-	-
H14	-
H15	454 nt T→C, 466 nt G→A	-
H16	177-234 nt deletion	-
H17	737 nt A→T, 740 nt A→G	-
H18	-	-
H19	-	-
H20	-	-
H21	462 nt G→T	-
H22	-	-
H23	-	-
H24	-	-
H25	177-234 nt deletion,	-
	660 nt A→G
H26	177-234 nt deletion	-
H27	-	-
H28	613 nt A→T	-
H29	-	-
H30	-	-

Table 2 Mutation analysis of polβ in low-incidence areas

Specimen	Gene mutation
	Carcinoma	Corresponding adjacent normal tissue
N1	670 nt A→G	–
N2	660 nt A→G	–
N8	660 nt A→G	–
N16	613 nt A→T	–
N22	670 nt A→G	–

Table 3 Gene and amino acid variations in polβ

Mutation code	Gene variation	Amino acid variation	Mutation
1	375 nt A→G	88 nt Ile→Val
2	454 nt T→C	114 nt Phe→Ser
3	462 nt G→T	117 nt Glu→termination	Termination
		codon	mutation
4	466 nt G→A	118 nt Gly→Glu
5	613 nt A→T	167 nt Lys→Ile
6	648 nt G→C	179 nt Gly→Arg
7	660 nt A→G	183 nt Arg→Gly
8	665 nt T→C	184 nt Gly→Gly	Synonymous
			mutation
9	670 nt A→G	186 nt Glu→Gly
10	737 nt A→T	208 nt Pro→Pro	Synonymous
			mutation
11	740 nt A→G	209 nt Lys→Lys	Synonymous
			mutation
12	177–234 nt deletion		Frameshift
			mutation
			Termination
			mutation