Frequencies of the expression of main protein antigens from Helicobacter pylori isolates and production of specific serum antibodies in infected patients

Table 1 Distribution of H pylori isolation rates in different types of gastric and duodenal diseases.

Types of diseases	Tested cases	H pylori isolation positve cases	Positive rate (%)
Chronic gastritis	88	68	77.3
Superficial	58	48	82.8
Active	15	10	75
Atrophy	15	10	75
Peptic ulcer	69	57	82.6
Gastric	16	12	77.3
Duodenal	46	39	84.7
Gastric + Duodenal	7	6	85.7
Total	157	125	79.6

Table 2 Detection results for the expressions of UreB, VacA, CagA1, HpaA, NapA, FlaA and FlaB in H pylori isolates.

Antigens	Cases (n)	Positive standard values (A490)	Value range of samples (A490)	Positive cases (n)	Positive rates (%)
UreB	125	0.25	0.47-1.93	125	100
VacA	125	0.21	0.27-1.73	82	65.6
CagA1	125	0.54	0.55-0.97	116	92.8
HpaA	125	0.34	0.52-1.94	125	100
NAP	125	0.36	0.36-1.23	117	93.6
FlaA	125	0.34	0.36-2.01	125	100
FlaB	125	0.3	0.31-1.78	124	99.2

Table 3 Detection results for the antibodies against UreB, VacA, CagA1, HpaA, NapA, FlaA and FlaB in sera of the patients.

Antibodies	Cases (n)	Positive standard value (A490)	Value range of samples (A490)	Positve cases (n)	Positive rates (%)
Anti-UreB	125	0.25	0.27-1.97	125	100
Anti-VacA	125	0.63	0.63-1.21	53	42.4
Anti-CagA1	125	0.46	0.56-1.05	112	89.6
Anti-HpaA	125	0.28	0.32-1.96	102	81.6
Anti-NAP	125	0.43	0.54-1.38	117	93.6
Anti-FlaA	125	0.44	0.52-1.76	123	98.4
Anti-FlaB	125	0.25	0.26-1.15	116	92.8

Table 4 Distribution of the expressions of seven antigens in H pylor strains isolated from the biopsies and the specific antibodies in sera of H pylori-infected patients.

Diseases	Strains/ Patients (n)	Antigen positive (n) / antibody positive (n)
		UreB	VacAabc	CagA1	HpaA	NapAdf	FlaA	FlaB
Chronic gastritisa	68/68	68/68	35/22	62/59	68/56	61/61	68/66	67/60
Superficialf	48/48	48/48	23/18	45/43	48/41	45/45	48/48	47/44
Activeb,c,d	10/10	10/10	9/2	9/9	10/8	10/10	10/10	10/9
Atrophy	10/10	10/10	3/2	8/7	10/7	6/6	10/8	10/7
Peptic ulcer	57/57	57/57	47/31	54/53	57/46	56/56	57/57	57/56
Gastric	12/12	12/12	9/6	11/11	12/10	11/11	12/12	12/11
Duodenal	39/39	39/39	32/21	38/37	39/31	39/39	39/39	39/39
Gastric+Duodenal	6/6	6/6	6/4	5/5	6/5	6/6	6/6	6/6
Total	125/125	125/125	82/53	116/112	125/102	117/117	125/123	124/116

The expression of VacA: ^bP<0.01 vs peptic ulcer, ^aP<0.05 vs superficial gastritis, ^dP<0.01 vs atrophy gastritis. The expression of NapA: ^cP<0.05 vs atrophy gastritis, ^fP<0.01 vs atrophy gastritis.