Excretion and detection of SARS coronavirus and its nucleic acid from digestive system

Table 1 Concentration and detection of SARS-CoV in 2 500-mL sewage before disinfection in Xiao Tang Shan Hospital ¹

Date	Cell cult2	Concentrate +PCR3	Inoculated cells+PCR4	Entero.5 PCR
10 June	-	+	-	-
11 June	-	+	-	-
12 June	-	+	-	-
13 June	-	+	-	-
14 June	-	+	-	-
15 June	-	+	-	-

¹Glass column diameter: 19 mm, bed height: 14 cm, eluate volume: 500 mL;

²Cell culture was maintained for 14 d to observe the cytopathic effect;

³PCR template was from the concentrates;

⁴PCR template was from the cultured cells;

⁵Enteroviruses were detected by general primer RT-PCR for enteroviruses.

Table 2 Concentration and detection of SARS-CoV in 2 500-mL sewage before disinfection in 309 Hospital of PLA ¹

Date	Cell cult	Concentrate +PCR	Inoculated cells+PCR	Entero. PCR
11 June	-	+	-	-
12 June	-	+	-	-
13 June	-	+	-	-
14 June	-	+	-	-
15 June	-	+	-	-
16 June	-	+	-	-

¹All explanatory notes are same as in Table 1.

Table 3 Concentration and detection of SARS-CoV in 25 000- or 50 000-mL sewage after disinfection in Xiao Tang Shan Hospital ¹

Date	Cell culture	Concentrate +PCR	Inoculated cells+PCR	Enterovirus PCR
11 June	-	-	-	-
12 June	-	-	-	-
13 June	-	-	-	-
14 June	-	-	-	-
15 June	-	-	-	-

¹All explanatory notes are same as in Table 1.

Table 4 Concentration and detection of SARS-CoV in 25 000-mL sewage after disinfection in 309 Hospital of PLA ¹

Date	Cell culture	Concentrate +PCR	Inoculated cells+PCR	Enterovirus PCR
11 June	-	+	+	-
12 June	-	-	-	-
13 June	-	+	+	-
14 June	-	-	-	-
15 June	-	+	+	-

¹All explanatory notes are same as in Table 1.