Genetic alterations of hepatocellular carcinoma by random amplified polymorphic DNA analysis and cloning sequencing of tumor differential DNA fragment

Table 1 Correlation between clinicopathological features and genetic alterations in hepatocellular carcinoma

Clinicopathological features		No.	P1	P2	P3	P4	P5	P6	P7	P8	P9	P10
Sex	M	43	14	14	7	20	19	9	18	10	10	22
	F	13	3	5	3	7	3	6	5	4	3	7
Age (yr)	< 50	24	6	12	6	11	8	7	9	8	5	14
	50	32	11	7	4	16	14	8	14	6	8	15
Serum AFP	< 20	21	3	6	5	9	5	7	9	9	6	8
level (µg/L)	20	35	14	13	5	18	17	8	14	5	7	21
HBsAg	Positive	39	17	12	8	24	16	10	13	13	8	27
	Negative	17	0b	7	2	3a	6	5	10	1a	5	2b
Tumor	3	12	3	3	7	4	5	2	6	3	3	7
size (cm)	> 3	44	14	16	3a	23	17	13	17	11	10	22
Histological	Well	20	0	4	7	5	8	5	10	7	3	13
grade	Moderately	28	12b	12	2	17a	12	8	10	5	8	13
	Poorly	8	5b	3	1	5a	2	2	3	2	2	3
Liver	Absent	9	2	3	3	3	4	3	5	1	3	4
cirrhosis	present	47	15	16	7	24	18	12	18	13	10	25
Tumor	Absent	11	5	3	0	6	5	2	5	1	2	4
capsule	Not intact	31	9	13	4a	16	10	11	13	10	7	17
	Intact	14	3	3	6a	5	7	2	5	3	4	8
Intrahepatic	Not	11	3	5	7	2	5	2	5	3	5	7
metastasis	observed
	Observed	45	14	14	3b	25a	17	13	18	11	8	22
Total [n (%)]		56	17 (30.4)	19 (33.9)	10 (17.9)	27 (48.2)	22 (39.3)	15 (26.8)	23 (41.1)	14 (25)	13 (23.2)	28 (50)

^aP < 0.05,

^bP < 0.01, χ² test.