Activation of transcription factors NF-kappaB and AP-1 and their relations with apoptosis-associated proteins in hepatocellular carcinoma

Table 1 Detection of NF-κB and AP-1 in HCC

Group	Cases	NF-κB	AP-1
		nucleus/cytoplasm	nucleus/cytoplasm
		+ nucleus (+) (%)	+ nucleus (+) (%)
Tumor tissue	40	22 (55)a	24 (60)
Well differentiated	25	13 (52)	14 (56)
Poorly differentiated	15	9 (60)	10 (66.7)
Nontumor tissue	40	7 (17.5)	4 (10)
Normal tissue	7	0	0

^aP < 0.05 vs tumor tissue.

Table 2 Expression of Fas, FasL, ICH-1L, and ICH-1S in HCC

Group	Cases	Fas (%)	FasL (%)	ICH-1L (%) ICH-1S (%)
Tumor tissue	40	7 (17.5)a	6 (15)	15 (37.5)	21 (52.5)
Nontumor tissue	40	21 (52.5)1	18 (45)2	29 (72.5)3	31 (77.5)4
Normal tissue	7	05	0b	2	16

^aP < 0.05 vs tumor tissue.

¹χ² = 10.2692,

²χ² = 8.5714,

³χ² = 9.8990,

⁴χ² = 5.4945,

⁵χ² = 4.6890,

⁶χ² = 8.2398.

Table 3 Relationship between activated NF-κB and apoptosis proteins in HCC

NF-κB	Cases	Apoptosis protein
		Fas		FasL		ICH-1L		ICH-1S
		+	–	+	–	+	–	+	–
Activated	22	6	16	5	17	13	12	17	8
Inactivated	18	1	17	1	17	2	13	4	11
χ2		1.9048		1.4495		4.4444		4.8722
P		> 0.05		> 0.05		< 0.05		< 0.05

Table 4 Relationship between activated AP-1 and apoptosis proteins in HCC

AP-1	Cases	Apoptosis protein
		Fas		Fas L		ICH-1L		ICH-1S
		+	–	+	–	+	–	+	–
Activated	25	7	18	6	19	13	12	16	9
Inactivated	15	0	15	0	15	2	13	3	12
χ2		3.3362		2.5621		4.4444		5.6207
P		> 0.05		> 0.05		< 0.05		< 0.025

Table 5 Relationship between activated NF-κB and apoptosis proteins in nontumor tissues

NF-κB	Cases	Adjacent nontumor tissues						ICH-1S		Cases	Normal liver tissues
		Fas		FasL		ICH-1L					Fas		FasL		ICH-1L		ICH-1S
		+	–	+	–	+	–	+	–		+	–	+	–	+	–	+	–
Activated	7	15	7	13	9	19	3	21	1	0	0	0	0	0	0	0	0	0
Inactivated	33	6	12	5	13	10	8	10	8	7	0	7	0	7	2	5	1	6
χ2		4.8211		3.9221		4.7130		6.8946			0		0		0		0
P		< 0.05		< 0.05		< 0.05		< 0.05			> 0.05		> 0.05		> 0.05		> 0.05