Cell cycle arrest and apoptotic cell death in cultured human gastric carcinoma cells mediated by arsenic trioxide

Table 1 The effects of As₂O₃ on the growth of MKN45 cells at different times and concentrations.

Groups		Cell number (1×105/mL)
		24 h	48 h	72 h
PBS control		7.15±1.08	9.18±0.99	13.34±0.54
As2O3 (µmol/L)	1	2.47±0.99	3.34±1.02	2.73±1.38
	5	2.51±0.63	2.84±1.12	1.75±1.12
	10.0	2.09±0.67	1.34±0.51	0.25±0.24

Table 2 Cytotoxic effect of As₂O₃ on MKN45 cells (%).

	Treatment time (t/h)
	24 h	48 h	72 h
1	11.5±5.2	18.9±5.6	34.8±6.8
As2O3 (µmol/L) 5	19.1±3.8	34.3±3.7	46.9±7.6
10.0	23.9±7.3	48.8±8.2	69.5±10.6

Table 3 Effect of As₂O₃ on apoptosis of MKN45 cells.

Groups		Apoptosis (%)
		24 h	48 h	72 h
Control		0.5±0.1	0.4±0.3	0.5±0.2
As2O3 (mmol/L)	1	2.7±2.1a	7.2±2.5a	12.3±3.6a
	5	7.3±5.7a	17.3±5.7a	21.1±5.6a
	10.0	11.6±3.4a	25.4±7.1a	35.8±8.1a

^aP<0.05 vs control.

Table 4 Effect of As₂O₃ on cell cycle of MKN45 cells.

Group		Cell cycle (%)
		24 h			48 h			72 h
		G1/G0	S	G2/M	G1/G0	S	G2/M	G1/G0	S	G2/M
Control		54.5	25.8	19.7	56.7	30.1	13.2	55.5	28.1	16.4
As2O3 (µmol/L)	1	55.6	28.1	16.3	37.2	40.2	22.6a	35.1	36.8	28.1a
	5	57.1	26.2	16.7	19.9	38.6	41.5a	18.4	35.2	46.4a
	10.0	55.2	27.3	17.5	13.1	17.9	69.0a	10.9	15.2	73.9a

^aP<0.05 vs control.