Relationship between Epstein-Barr virus-encoded proteins with cell proliferation, apoptosis, and apoptosis-related proteins in gastric carcinoma

Table 1 Sequence and co-ordinate of primers and probes for RT-PCR analysis.

Transcript		Oligonucleotide sequence (5’-3’)	Product size (bp)	Genome coordinate
EBNA1	5’ primer	GATGAGCGTTTGGGAGAGCTGATTCTGCA	273	67510-67539
	3’ primer	TCCTCGTCCATGGTTATCAC		108075-108056
	probe	AGACCTGGGAGCAGATTCAC		67608-67627
EBNA2	5’ primer	GCTGCTACGCATTAGAGACC	339	47892-47911
	3’ primer	TCCTGGTAGGGATTCGAGGG		48616-48597
	probe	CAGCACTGGCGTGTGACGTGGTGTAAGTT		48391-48420
LMP1	5’ primer	TCCTCCTCTTGGCGCTACTG	490	169383-169364
	3’ primer	TCATCACTGTGTCGTTGTCC		168740-168759
	probe	GAACAGCACAATTCCAAGGAACAATGCCTG		169061-169090
BZLF1	5’ primer	ATTGCACCTTGCCGCCACCTTTG	608	103194-103180
	3’ primer	CGGCATTTTCTGGAAGCCACCCGA		102486-102463
	probe	CACTGCTGCTGCTGTTTGAACAGT		102772-102795
BARF1	5’ primer	GGCTGTCACCGCTTTCTTGG	203	165560-165579
	3’ primer	AGGTGTTGGCACTTCTGTGG		165762-165743
	probe	CTGGTTTAAACTGGGCCCAGGAGAGGAGCA		165644-165673
BHRF1	5’ primer	GTCAAGGTTTCGTCTGTGTG	211	53830-53849
	3’ primer	TTCTCTTGCTGCTAGCTCCA		54480-54461
	probe	ATGCACACGACTGTCCCGTATACAC		54435-54411

Table 2 Comparison of clinicopathological data between EBVaGC and EBVnGC patients.

	EBVaGC (n = 13)	EBVnGC (n = 45)	P
Age (yr) (mean±SD)1	59.76±10.17	57.31±14.05	0.5613 (t = 0.5844)
Sex
Male	13	35	0.1466 (χ2 = 2.1070)
Female	0	10
Tumor location
Cardia/body	9	21	0.1516 (χ2 = 2.0566)
Antrum	4	24
Histological subtype
Adenocarcinoma	12	41	0.6704 (χ2 = 0.1811)
Signet ring carcinoma	1	4
Tumor differentiation
Moderate	1	7	0.7890 (χ2 = 0.0716)
Poor	12	38
Lymph node metastasis
Present	10	33	0.9999 (χ2 = 0.0098)
Absent	3	12
Tumor stage2
Early	0	1	1.0000
Advanced	13	44

¹Age was compared using Student’s t test;

²Tumor stage was compared using two-tailed Fisher’s exact test; the remainders, using χ²-test.

Table 3 Apoptotic index and ki-67 index in EBVaGC and EBVnGC (mean±SD).

	AI	KI
EBVaGCs (n = 13)	0.97±0.411	28.25±6.282
EBVnGCs (n = 45)	2.03±0.60	37.86±14.52

¹t = 5.9795, P = 0.00001<0.01;

²t’ = 3.4579, P = 0.0012<0.01.

Table 4 bcl-2 and p53 expression in EBVaGC and EBVnGC.

	n	bcl-2		p53 expression		p53 overexpression
		+	-	+	-	+	-
EBVaGC	13	7	6	11	2	2	11
EBVnGC	45	22	23	39	6	26	19
χ2		0.0991		0.0716		7.2593
P value		0.7529		0.7912		0.0085