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©2005 Baishideng Publishing Group Co.
World J Gastroenterol. Jan 14, 2005; 11(2): 285-288
Published online Jan 14, 2005. doi: 10.3748/wjg.v11.i2.285
Published online Jan 14, 2005. doi: 10.3748/wjg.v11.i2.285
Table 1 Primers of PTEN microsatellite loci and exons.
| Loci | Position | Primer sequence |
| D10S215 | 10q22-23 | P1 TGGCATCATTCTGGGGA |
| P2 TTACGTTTCTTCACATGGT | ||
| D10S541 | 10q22-23 | P1 AAGCAAGTGAAGTCTTAGAACCACC |
| P2 CCACAAGTAACAGAAAGCCTGTCTC | ||
| D10S2491 | 10q23-23 | P1 TTATAAGGACTGAGTGAGGGA |
| P2 GTTAGATAGAGTACCTGCACTC | ||
| Exon 5 | P1 CTTATTCTGAGGTTATCTTTTTTACC | |
| P2 CTCAGAATCCAGGAAGAGGA | ||
| Exon 8 | P1 ACACATCACATACATACAAGTC | |
| P2 GTGCAGATAATGACAAGGAATA |
Table 2 PTEN LOH in gastric cancer and precancerous lesion.
| Lesions | D10S215 % (n) | D10S541 % (n) | D10S2491 % (n) | Total % |
| AG | 3.3 (1/30) | 3.3 (1/30) | 6.7 (2/30) | 10 |
| IM | 3.3 (1/30) | 3.3 (1/30) | 3.3 (1/30) | 10 |
| AH | 6.7 (2/30) | 3.3 (1/30) | 6.7 (2/30) | 13.3 |
| Gastric cancer | ||||
| Early stage | 6.7 (2/30) | 10 (3/30) | 6.7 (2/30) | 20 |
| Advanced stage | 13.3 (4/30) | 6.7 (2/30) | 13.3 (4/30) | 30 |
Table 3 Mutation of PTEN in gastric cancer.
| Case | Exon | LOH | Coden | Base change | Stage | Pathology |
| 11 | 5 | + | 91 | GAA to CAA | IIIa | Signet-ring cell cancer |
| 19 | 8 | + | 335 | CGA to TGA | II | Hypodifferentiated adenocarcinoma |
| 22 | 8 | + | 329 | Del 4 bp | IIIb | Undifferentiate gastric cancer |
- Citation: Li YL, Tian Z, Wu DY, Fu BY, Xin Y. Loss of heterozygosity on 10q23.3 and mutation of tumor suppressor gene PTEN in gastric cancer and precancerous lesions. World J Gastroenterol 2005; 11(2): 285-288
- URL: https://www.wjgnet.com/1007-9327/full/v11/i2/285.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i2.285