Screening of stimulatory effects of dietary risk factors on mouse intestinal cell kinetics

Table 1 Proximate composition of dried pulp of tamarind fruit.

Constituents	Proximate composition of the dried pulp powder (%)1
Moisture	3.5-8.8
Protein	1.7-2.4
Total sugars	15.8-25.0
Ash	2.1-3.2
Free tartaric acid	8.7-11.1
Starch	20-41.3

¹Source: Reference[14].

Table 2 Composition of the diet administered to control and experimental animals.

Constituents	Control diet	Experimental diet
Caesin (%)	23.8	23.8
DL- methionine (%)	0.5	0.5
Corn starch (%)	30	22.7
Sucrose (%)	30	27.5
Cellulose (%)	6.5	6.5
Corn oil (%)	5	5
Salt mix (%)	3	3
Vitamin mix (%)	1	1
Choline bitartrate (%)	0.2	0.2
TI (10%)1- mixed
Sucrose (%)	-	2.5
Tartaric/Malic/Citric acid (%)	-	3.3
Starch (%)	-	4
Animal fat (%)2 - mixed	-	10
Cholesterol (%)	-	2

¹TI was mixed (10%) in the total diet and compensated with corn starch and sucrose of the control diet for the nutrients composition for preparing experimental diet ²Choline bitartrate and corn oil were excluded from the dietary regimens of groups3 and 4.

Table 3 Activities of succinate dehydrogenase, acid-B- galactosidase and dipeptidyl amino peptidase IV (values are expressed as absorbance units, mean±SE; n = 6).

Enzymes	Crypt segments	CD	TI	TIFB	OB
SDH	Luminal epithelium	0.596±0.053	0.533±0.038	0.512±0.053a	0.524±0.067a
DAP IV	Luminal epithelium	0.178±0.027	0.163±0.008	0.156±0.019	0.125±0.014a
	Basal crypt epithelium	0.196±0.012	0.179±0.003	0.162±0.005	0.151±0.030
Acid-b-Gal	Luminal epithelium	0.526±0.046	0.443±0.035	0.381±0.028a	0.314±0.029a
	Basal crypt epithelium	0.592±0.061	0.527±0.036	0.493±0.058	0.397±0.011b
	Apical crypt epithelium	0.863±0.067	0.639±0.057c	0.587±0.009c	0.452±0.065c

^aP<0.05 vs CD in the crypt segment of luminal epithelium; ^bP<0.01 vs CD in the crypt segment of basal crypt epithelium; ^cP<0.05 vs CD in the crypt segment of apical crypt epithelium.