Increased activity of Pgp multidrug transporter in patients with Helicobacter pylori infection

Table 1 Effect of sample storage.

H pylori positivepatients	D1			D2
	Rh123 MF	Rh123+CsA MF	RMF	Rh123 MF	Rh123+CsA MF	RMF
1	73.14	124.58	1.703	25.00	44.76	1.790
2	75.25	17.86	0.237	27.83	4.28	0.157
3	5.47	20.85	3.404	17.25	41.72	2.419
4	83.40	113.79	1.364	43.03	61.20	1.423
5	83.40	113.79	1.364	90.44	172.18	1.920
6	141.21	68.47	0.485	72.91	80.63	1.106
7	72.31	223.19	3.087	46.72	223.44	4.783
8	262.30	220.78	0.842	308.4	326.90	1.06
9	135.53	93.90	0.693	36.71	255.95	6.972
10	170.21	225.35	1.324	220.95	223.71	1.012
11	115.20	165.20	1.434	37.88	94.26	2.288
12	11.30	25.10	2.221	25.76	134.78	5.332
RMF (mean±SD)	1.427±1.034			2.522±2.067

D 1 = fresh cells, several hours of sampling; d 2 = cells stored overnight at room temperature; Rh123 = rhodamine; CyA = cyclosporine A; MF = mean fluorescence; RMF = ratio of mean fluorescence.

Table 2 Role of pretherapeutic determination of Pgp activity.

Variables	Therapy cycle			B/A and controls
	H pylori positive		H pylorinegative
	1 = A (n = 20)	2 or more = B (n = 33)	NO (n = 12)	Pa	Beta Pb
Age (yr)	47.5±8.8	47.3±13.7	48.3±16.5	0.973	0.152
Sex (M/ F)	9/11	15/18	5/7	0.975	0.153
DHP	2.75±0.91	2.90±0.97	1.0±0.0	0.00001	0.00065
DIEn	2.65±0.81	3.06±0.86	1.0±0.0	0.00001	0.45
RMF (mean±SD)	0.889±0.28	1.647±0.65	0.787±0.29	0.00001	0.000005

H pylori positive patients: A = one therapy cycle, B = two or more therapy cycles; NO = no therapy, M = male; F = female; DHP = H pylori density; DIEn = density of intraepithelial neutrophil infiltration; RMF = ratio of mean fluorescences, Pa for analysis of variance, Pb for multiple regression analysis.

Table 3 Impact of initial values on H pylori eradication efficacy.

Variable	Final eradication of H pylori		P
	Successful (n = 31)	Unsuccessful (n = 22)
Age (yr)	46.54±12.51	48.54±11.44	0.556
Sex (M/F)	15/16	9/13	0.598
DHP	2.90±0.97	2.77±0.92	0.626
DIEn	2.77±0.846	3.09±0.86	0.189
RMF (mean±SD)	1.26±0715	1.54±0.66	0.13418

M = male; F = female; DHP = H pylori density according to Sydney criteria; DIEn = density of intraepithelial neutrophil infiltration according to Sydney criteria; RMF = ratio of means.