Co-administration of cyclosporine A alleviates thioacetamide-induced liver injury

Table 1 Primer sequence and expected lengths of fragments in RT-PCR analysis of rat liver selected genes and β-actin.

Gene		Primer sequence	Expected lengths of fragments
β-actin	Forward	5- GTCTTCCCCTCCATCGTG-3	992 bp
	Reverse	5-TGCTTGCTGATCCACATCTG-3’
FGFR2	Forward	5-GGACAGACCCAAGGAGGCAG-3	667 bp
	Reverse	5-GCCAGCAGTCCCTCATCATC-3’
FGFR4	Forward	5-GGAGGTGCTGTATCTGAGGAACGTG-3	599 bp
	Reverse	5-TGTCGGAGGCATTGTCTTTCAG-3’
TGFβ R1	Forward	5-CGTCGCTGCCTGCTTCTCATC-3	646 bp
	Reverse	5-CCGCCATTTGCCTCGCC-3’
TGFβ R2	Forward	5-CGACAACTGCGCCATCATCC-3	649 bp
	Reverse	5-GGCCATGTATCTCGCTGTTCCC-3’
TGFβ R3	Forward	5-GGTGTGGCATCTGAAGACGGAG-3	787 bp
	Reverse	5-GCTCAGGAGGAATGGTGTGGACT-3’
Collagen1	Forward	5- GCGAAGGCAACAGTCGATTC-3’	69 bp
	Reverse	5-CCCAAGTTCCGGTGTGACTC-3’
Collagen3	Forward	5-CAGCTGGCCTTCCTCAGACTT-3’	70 bp
	Reverse	5-GCTGTTTTTGCAGTGGTATGTAATG-3’
Abcb1	Forward	5-GGACCCACAGGCGGAGG-3	634 bp
	Reverse	5-GCAGGGTTGTTGTAGGGCTCA-3’

Table 2 Antibodies used in current investigation.

Antibody1	Dilution	Protein size (ku)
β-actin, mouse polyclone	0.3888889	41
FGFR-2,rabbit polyclone, sc-122	0.3888889	119
FGFR-4, rabbit polyclone, sc-124	0.3888889	93
TGFβR1, rabbit polyclone, sc-402	1:1000	53

¹All antibodies were purchased from Santa Cruz Biotechnology (CA, USA).