Copyright
©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Jan 7, 2005; 11(1): 61-68
Published online Jan 7, 2005. doi: 10.3748/wjg.v11.i1.61
Published online Jan 7, 2005. doi: 10.3748/wjg.v11.i1.61
Gene | cDNA primer sequence | Annealing temperature (°C) | Product size (bp) |
TGF-b1 | Forward: 5’-GCGACTCGCCAGAGTGTTAT-3’ | ||
Reverse: 5’-ATAGTTGGTGTCCAGGGCTCG-3’ | 61 | 284 | |
TbRI | Forward: 5’-ACGGCGTTACAGTGTTCTG-3’ | ||
Reverse: 5’-CCACACCGTCTATATCTGG-3’ | 57 | 358 | |
TbRII | Forward: 5’-AGCAACTGCAGCATCACCTC-3’ | ||
Reverse: 5’-ACTACAGACTCTTCTACAGG-3’ | 57 | 688 | |
Smad2 | Forward: 5’-ATCCTAACAGAACTTCCGCC-3’ | ||
Reverse: 5’-GAGTCGTTTTTGAAGGGGTG-3’ | 57 | 489 | |
Smad3 | Forward: 5’-GGAGGGCAGGCTTGGGGAAAATG-3’ | ||
Reverse: 5’-CCCCTCCCCCGGCCACCACATTAT-3’ | 57 | 284 | |
Smad4 | Forward: 5’-AAGGTGAAGGTGATGTTTG-3’ | ||
Reverse: 5’-CTCGATAAGGTGGATGACTA-3’ | 56 | 264 | |
Smad7 | Forward: 5’-GTGGGGAGGCTCTACTGTGTC-3’ | ||
Reverse: 5’-CAGCTTTCGGAACTACCTCTTTGG-3’ | 57 | 294 | |
p15 | Forward: 5’-TTCTTTAAATGGCTCCACCTGCCT-3’ | ||
Reverse: 5’-AACCGTCGGAAGTAGCTTAATCCA-3’ | 60 | 436 | |
p21 | Forward: 5’-AGTGGACAGCGAGCAGCTGA-3’ | ||
Reverse: 5’-ATCTTTAGACAGTACGACCAGAC-3’ | 58 | 380 |
Time of TGF-b1treatment (h) | Cell cycle (%) | |||
G0-G1 | S | G2-M | Apoptosis | |
0 | 52.72 | 32.39 | 14.89 | 0.34 |
24 | 57.07 | 33.82 | 9.11 | 6.61 |
48 | 60.2 | 29.54 | 10.26 | 9.86 |
- Citation: Li X, Zhang YY, Wang Q, Fu SB. Association between endogenous gene expression and growth regulation induced by TGF-β1 in human gastric cancer cells. World J Gastroenterol 2005; 11(1): 61-68
- URL: https://www.wjgnet.com/1007-9327/full/v11/i1/61.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i1.61