Gastric Cancer
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Jan 7, 2005; 11(1): 61-68
Published online Jan 7, 2005. doi: 10.3748/wjg.v11.i1.61
Table 1 Oligonucleotide primers for RT-PCR (Sangon, Shanghai, China).
GenecDNA primer sequenceAnnealing temperature (°C)Product size (bp)
TGF-b1Forward: 5’-GCGACTCGCCAGAGTGTTAT-3’
Reverse: 5’-ATAGTTGGTGTCCAGGGCTCG-3’61284
TbRIForward: 5’-ACGGCGTTACAGTGTTCTG-3’
Reverse: 5’-CCACACCGTCTATATCTGG-3’57358
TbRIIForward: 5’-AGCAACTGCAGCATCACCTC-3’
Reverse: 5’-ACTACAGACTCTTCTACAGG-3’57688
Smad2Forward: 5’-ATCCTAACAGAACTTCCGCC-3’
Reverse: 5’-GAGTCGTTTTTGAAGGGGTG-3’57489
Smad3Forward: 5’-GGAGGGCAGGCTTGGGGAAAATG-3’
Reverse: 5’-CCCCTCCCCCGGCCACCACATTAT-3’57284
Smad4Forward: 5’-AAGGTGAAGGTGATGTTTG-3’
Reverse: 5’-CTCGATAAGGTGGATGACTA-3’56264
Smad7Forward: 5’-GTGGGGAGGCTCTACTGTGTC-3’
Reverse: 5’-CAGCTTTCGGAACTACCTCTTTGG-3’57294
p15Forward: 5’-TTCTTTAAATGGCTCCACCTGCCT-3’
Reverse: 5’-AACCGTCGGAAGTAGCTTAATCCA-3’60436
p21Forward: 5’-AGTGGACAGCGAGCAGCTGA-3’
Reverse: 5’-ATCTTTAGACAGTACGACCAGAC-3’58380
Table 2 Cell cycle alterations of BGC-823 cells treated with TGF-β1.
Time of TGF-b1treatment (h)Cell cycle (%)
G0-G1SG2-MApoptosis
052.7232.3914.890.34
2457.0733.829.116.61
4860.229.5410.269.86