Association of low p16INK4a and p15INK4b mRNAs expression with their CpG islands methylation with human hepatocellular carcinogenesis

Table 1 CpG island methylation status and expression of p16 and p15 genes and clinical information of 20 HCC patients

Cases	p16 methylation1	p16 expression	p15 methylation1	p15 expression	HBsAg4	Cirrhosis5	Differentiation6
1	±	D	+	D	+	Y	L
2	+	D	+	D	+	Y	L
3	±	D	+	D	+	N	M
4	±	N	±	D	+	Y	M
5	±	D	+	D	+	N	L
6	+	D	-	N	+	N	L
7	-	N	-	N	-	N	H
8	±	N	±	D	-	N	M
9	±	D	±	D	+	Y	M
10	-	N	-	N	-	N	H
11	+	D	N2	N	+	Y	L
12	±	D	-	I	+	Y	M
13	-	D	±	D	+	Y	M
14	-	N	-	I	-	N	M
15	+	D	-	N	+	N	L
16	±	N	±	D	-	Y	L
17	-	D	-	N	-	N	H
18	-	I	-	I	+	N	H
19	±	D	±	D	-	Y	L
20	-	N	-	N	+	N	H

¹+Positively amplified with methylation primers only; - positively amplified with unmethylation primers only; ± positively amplified with methylation and unmethylation primers; ²N: Negatively amplified with methylation or unmethylation primers, ³D: Decreased; I: Increased; N: No significant difference; compared with non-cancer tissue,

⁴+: Positive; -: Negative;

⁵Y: Cirrhosis; N: No cirrhosis,

⁶H: High differentiation; M: moderate differentiation; L: Low differentiation.