Protein kinase C-dependent activation of P44/42 mitogen-activated protein kinase and heat shock protein 70 in signal transduction during hepatocyte ischemic preconditioning

Table 1 Changes in serum concentration of ALT and AST (n = 6, mean ± SD)

Group	AST (U/L)	ALT (U/L)
C	99.3 ± 13.2	61.90 ± 12.1
IR	820.9 ± 111.3d	762.80 ± 130.5d
IP	407.7 ± 73.7b	281.00 ± 35.6b
IR + PMA	553.2 ± 58.67b	354.37 ± 53b
IP + PD	732.9 ± 91.1f	466.20 ± 82.8f
IP + CHE	678.6 ± 136.5f	645.61 ± 90.4f

^bP < 0.01 versus group IR,

^dP < 0.01 versus group C,

^fP < 0.01 versus group IP.

Table 2 Protection of hypoxic preconditioning against cytotox-icity caused by hypoxic hepatocyte incubation (n = 6, mean ± SD)

Group	Hepatocyte viability (%)
C	95 ± 10.8
HR	35.57 ± 3.99b
HP	81.49 ± 12.1d
HR + PMA	75.29 ± 11.9d
HP + PD	51.03 ± 9.09f
HP + CHE	47.21 ± 5.42f

^bP < 0.01 vs group C,

^dP < 0.01 vs group HR,

^fP < 0.01 vs group HP.

Table 3 Changes in levels of phosphorylation activity of PKC in the liver (n = 6, mean ± SD)

Group	Phosphorylation activity of PKC (fmol/mg/min)
C	36.28 ± 3.8
IR	42.78 ± 2.22
IP	112.61 ± 4.86b
IP + CHE	34.07 ± 2.77d
IR + PMA	165.47 ± 27.25b

^bP < 0.01 versus group IR,

^dP < 0.01 versus group IP.

Table 4 Changes in PKC phosphorylation activity during hypoxia in control and preconditioned hepatocytes (n = 6, mean ± SD)

Group	Phosphorylation activityof PKC (fmol/mg/min)
C	32.67 ± 5.11
HR	42.63 ± 4.73
HP	109.42 ± 16.09b
HR + PMA	152.47 ± 19.59b
HP + CHE	65.28 ± 5.36d

^bP < 0.01 vs group HR,

^dP < 0.01 vs group IP.