Cryopreservation and gel collagen culture of porcine hepatocytes

Table 1 Nucleotide sequences of primers and band sizes

Primers		Sequences (5’-3’)	Band sizes
mRNA	sense	CTTATTCCAGGGGTCTGTTTC	324 bp
	antisense	TCGTTTCTCTCAGGCTCTTCT
G3PDH	sense	CATCATCCCTGCTTCTACCC	160 bp
	antisense	CCTGCTTCACCACTTTCTTG

Table 2 Viability and 24 hours attaching efficiency of thawed hepatocytes (%)

Viability/group (mL/L)	50 DMSO	100 DMSO	150 DMSO	200 DMSO
Viability	51 ± 3	71 ± 4a	83 ± 4c	79 ± 5c
Attaching efficiency	32 ± 6	70 ± 5a	81 ± 5c	77 ± 6a

n = 5,

^aP < 0.05 vs 50 mL/L or 100 mL/L DMSO group,

^cP < 0.05 vs 50 mL/L DMSO group.

Table 3 Change of albumin mRNA expression in culture pig hepatocytes (mean ± SD)

Band density/group	Mixed gel group	Sandwich group	Control group
Mean rate of density	0.44 ± 0.041a	0.353 ± 0.021c	0.261 ± 0.02

n = 3,

^aP < 0.05 vs group of control or sandwich;

^cP < 0.05 vs con-trol group.