Nuclear and mitochondrial DNA microsatellite instability in Chinese hepatocellular carcinoma

Table 1 Sequences of primer for PCR analysis

Repeat sequence	mtDNA region	Position	Annealing(°C)	Primer (5’-3’)
(C)n	270 - 425	D - loop	58	TCCACACAGACATCAATAACA
				AAAGTGCATACCGCCAAAAG
(CA)n	467 - 556	D - loop	55	CCCATACTACTAATCTCATCAA
				TTTGGTTGGTTCGGGGTATG
(C)6	3529 - 3617	ND1	55	CCGACCTTAGCTCTCACCAT
				AATAGGAGGCCTAGGTTGAG
(A)7	4555 - 4644	ND2	55	CCTGAGTAGGCCTAGAAATAAA
				ACTTGATGGCAGCTTCTGTG
(T)7	9431 - 9526	COIII	55	CCAAAAAGGCCTTCGATACG
				GCTAGGCTGGAGTGGTAAAA
(C)6 and (A)8	12360 - 12465	ND5	55	CACCCTAACCCTGACTTCC
				GGTGGATGCGACAATGGATT
(CCT)3 and (AGC)3	12940 - 13032	ND5	55	GCCCTTCTAAACGCTAATCC
				TCAGGGGTGGAGACCTAATT

Table 2 Relationship between MSI and clinical parameters

		n	mtMSI positive	mtMSI negative
Sex	Male	42	10	32
	Female	10	1	9
Age	< 30	1	1	0
	30 - 60	41	8	33
	≥ 60	10	2	8
HBsAg	Positive	38	8	30
	Negative	14	3	11
Cirrhosis	Positive	37	9	28
	Negative	15	2	13
AFP	Positive	26	6	20
	Negative	26	5	21