Induction of tryptase and histamine release from human colon mast cells by IgE dependent or independent mechanisms

Table 1 Effects of pertussis toxin and metabolic inhibitors on anti-IgE induced histamine and tryptase release from colonic mast cells

Treatment		Net mediator release (ng/mL)
		Tryptase	Histamine
Untreated		11.4 ± 2.7	14.0 ± 3.2
Pertussis toxin	0.1 μg/mL	3.2 ± 1.3a	5.4 ± 1.7a
	1.0 μg/mL	4.0 ± 1.6a	4.0 ± 1.9a
Metabolic inhibitors		-2.1 ± 1.7a	-2.6 ± 1.7a

The values shown aremean±SEMof four separate experiments performed in duplicate.

^aP < 0.05 compared with the untreated group (paired Students t test). Metabolic inhibitors=1 μMantimycin A+10 mM 2-deoxy-D-glucose.

Table 2 Effects of pertussis toxin and metabolic inhibitors on calcium ionophore induced tryptase and histamine release from colonic mast cells

Treatment		Mediator release (ng/mL)
		Tryptase	Histamine
Untreated		13.8 ± 2.4	34.3 ± 3.4
Pertussis toxin	0.1 μg/mL	4.0 ± 1.6a	2.7 ± 2.8a
	1.0 μg/mL	1.4 ± 1.8a	-0.1 ± 1.3a
Metabolic inhibitors		2.0 ± 1.4a	1.8 ± 2.6a

The values shown aremean±SEMof four separate experiments performed in duplicate.

^aP < 0.05 compared with the untreated group (paired Students t test). Metabolic inhibitors=1 μMantimycin A+10 mM 2-deoxy-D-glucose.