Basic Research
Copyright ©The Author(s) 2004.
World J Gastroenterol. Dec 15, 2004; 10(24): 3583-3589
Published online Dec 15, 2004. doi: 10.3748/wjg.v10.i24.3583
Table 1 p53 status, transduction efficacy and IC50 value of 5-FU in pancreatic cancer cell lines; mean ± SD
Cell linep53 statusβ-gal1%5-FU-IC50 value1μg/mL
Human
Capan-1159 GCC→GTC92 (4.5)8.25 (0.45)
Capan-2wild type95 (7.2)5.75 (0.55)
FAMPAC175 CGC→CAT82 (7.8)8.00 (0.45)
PANCL273 CGT→CAT82 (7.8)9.50 (0.75)
Murine rat
ASwild type73 (5.3)6.55 (0.45)
DSL6ANull83 (6.2)7.75 (0.55)
Table 2 In vitro growth of different pancreatic cancer cell lines after indicated treatment 1measured by WST-1 cell viability assay2; % mean (95%CI)
Cell lineControlAd-CD-controlAd-p535-FUAd-p53 + 5-FU
Human
Capan-197.8 (96.0-99.5)91.9 (86.6-97.6)55.0 (51.3-58.6)72.9 (70.4-74.4)30.7 (28.9-32.4)
Capan-297.9 (95.7-100.0)91.3 (88.2-94.5)75.0 (71.3-78.6)55.6 (53.1-58.0)51.2 (48.0-54.4)
FAMPAC98.0 (95.9-100.1)93.3 (90.5-96.2)48.6 (44.7-52.4)70.6 (68.0-73.1)31.7 (29.6-33.8)
PANCL98.4 (97.1-99.8)95.0 (92.2-97.8)64.7 (59.8-69.5)73.9 (71.6-76.2)33.8 (31.5-36.0)
Murine rat
AS99.0 (97.6-100.4)97.9 (95.7-100.0)92.3 (88.9-95.8)56.8 (52.2-61.3)53.1 (48.2-58.0)
DSL6A98.8 (97.5-100.0)94.0 (90.8-97.2)64.2 (60.5-67.9)69.4 (66.0-72.9)32.8 (29.3-36.2)