Real-time determination of human telomerase reverse transcriptase mRNA in gastric cancer

Table 1 Sequences of primers and probes used in this study

Gene and oligonucleotide	Sequence	PCR product size (bp)
GAPDH
Upper primer	5’-GAAGGTGAAGGTCGGAGTC-3’
Lower primer	5’-GAAGATGGTGATGGGATTTC-3’	226
Probe	5’-(FAM) CAAGCTTCCCGTTCTCAGCC (TAMRA)-3’
hTERT
Upper primer	5’-TGACACCTCACCTCACCCAC-3’
Lower primer	5’-CACTGTCTTCCGCAAGTTCAC-3’	95
Probe	5’-(FAM) ACCCTGGTCCGAGGTGTGTCCCTGA (TAMRA)-3’

The primers placed in different exons, were tested to ensure that they did not amplify the contaminated genomic DNA.

Table 2 Relationship between hTERT mRNA expression level and clinicopathological parameters in patients with gastric cancer

Parameter	n	NhTERT		P
		Low (n = 17)	High (n = 18)
Age (yr)
< 50	13	7	6
≥ 50	22	10	12	0.73
Gender
Male	25	13	12
Female	10	4	6	0.71
Size of diameter (cm)
< 5	19	11	8
≥ 5	16	6	10	0.31
Location
Cardia	14	8	6
Body and antrum Degree of differentiation	21	9	12	0.50
Well and moderately differentiated	16	12	4
Poorly differentiated and undifferentiated	19	5	14	0.007
Lymph node metastasis
N0	8	4	4
N1	13	8	5
N2	14	5	9	0.40
Depth of invasion
T1 and T2	24	13	11
T3 and T4	11	4	7	0.43
Metastasis
M0	20	12	8
M1	15	5	10	0.17