Effects of phosphorothioate anti-sense oligodeoxynucleotides on colorectal cancer cell growth and telomerase activity

Table 1 Selection of optimal concentration of PASODN

Clones	Concentration of PASODN (μmol/L)
	0	1	2.5	5	10	20
Mean	64.7	51.5	41.0	24.7	11.1	8.4
Inhibitive rate (%)	0	20.4	36.6	61.8	82.8	87.0

Table 2 Colony-forming efficiency in each group (mean ± SD)

	PASODN(10 μmol/L)	PMSODN(10 μmol/L)	PBS(same volume)
Mean±SD	41.2 ± 5.5	196.3 ± 13.5	202.8 ± 8.5
Efficiency (%)	10.3	49.1	50.7
vs PASODN		P < 0.01	P < 0.01

Table 3 Variation of telomerase activity (mean ± SD)

Group	SA	P
PASODN	0.18 ± 0.12
PMSODN	1.89 ± 0.24	< 0.01
PBS	1.98 ± 0.25	< 0.01

Table 4 Effect of PASODN on LS-174T cell cycle

Groups	G0/G1	G2/M	S
Control	49.91	21.79	28.30
PASODN	53.20	11.25	35.55