Hepatitis B virus genotypes, phylogeny and occult infection in a region with a high incidence of hepatocellular carcinoma in China

Table 1 Serological markers of 20 asymptomatic carriers with positive HBV DNA

No.	Age (yr)	Sex	HBsAg	Anti-HBs	Anti-HBc	HBeAg	Anti-HBe
601	18	Male	+	-	+	+	-
602	18	Male	+	-	+	+	-
603	28	Female	+	-	+	+	-
604	20	Male	+	-	+	+	-
605	27	Male	+	-	+	+	-
606	18	Male	+	-	+	+	-
607	31	Female	+	-	+	+	-
609	22	Female	+	-	+	+	-
610	19	Male	+	-	+	+	-
615	38	Male	+	-	+	+	-
618	22	Male	+	-	+	+	-
619	20	Male	+	-	+	+	-
621	24	Male	+	-	+	+	-
622	28	Male	+	-	+	+	-
623	30	Male	+	-	+	+	-
624	25	Male	+	-	+	+	-
629	28	Female	+	-	+	+	-
630	28	Female	+	-	+	+	-
633	18	Male	+	-	+	+	-
634	19	Male	+	-	+	+	-

Table 2 Serological markers of HBsAg-negative asymptomatic carriers with positive HBV DNA

Sample No.	HBsAg	Anti-HBs	Anti-HBs Titre (mIU/mL)	HBeAg	Anti-HBe	Anti-HBc
1	-	+	5.1	-	+	+
14	-	+	108.0	-	+	-
16	-	+	18.2	-	+	+
23	-	+	13.9	-	-	-
25	-	-	-	-	+	+
37	-	-	-	-	-	+

Table 3 PCR Primers

Primers	Sequence	Position (nt)
LSOB1	5’-GGCATTATTTGCATACCCTTTGG-3'	2739-2762
BPOLEO1	5’-CTGAGAGTCCAAGAGTCCTCT-3'	1657-1677
LSBI1	5’-TTGTGGGTCACCATATTCTT-3'	2809-2829
POLSEQ2	5’-AGCAAACACTTGGCATAGGC-3'	1168-1188
MDN5	5’-AGGAGGCTGTAGGCATAAAT-3'	1774-1794
BPOLEO1	5’-CTGAGAGTCCAAGAGTCCTCT-3'	1657-1677
BPOLBO2	5’-TCTTGTCTTACTTTTGGAAGA-3’	2216-2236
PSISEQ2	5’-GCTGTTCCGGAATTGGAGCC-3'	65-84
POLSEQ1	5’-ACCAAGCGTTGGGGCTACTC-3'	847-866
MDD2	5’-GAAGAATAAAGCCCAGTAAA-3'	2481-2500
POLSEQ6	5’-TTTCACTTTCTCGCCAACTTA-3'	1089-1109
MDC1	5’-TTGATAAGATAGGGGCATTTG-3'	2304-2324

Table 4 Sequencing primers

Primers	Sequence	Position (nt)
LSB1	5’-TTGTGGGTCACCATATTCTT-3'	2809-2829
PSISEQ2	5’-GCTGTTCCGGAATTGGAGCC-3'	65-84
ds2	5’-AGTCCGGTACGTCACCTTG-3'	3198-1
ds	5’-TACCGAAAATGGAGAACACA-3'	147-166
ADELN	5’-TAGTCCAGAAGAACCAACAAG-3'	432-453
MD14	5’-GCGCTGCAGCTATGCCTCATCTTC-3’	418-433
ADELP	5’-CCTGTATTCCCATCCCATCATC-3'	597-618
POLSEQ8	5’-TGTTTTCGAAAACTTCCTGT-3'	943-962
POLSEQ9	5’-ACAGGAAGTTTTCGAAAACA-3'	943-962
POLSEQ6	5’-TTTCACTTTCTCGCCAACTTA-3'	1089-1109
POLSEQ2	5’-AGCAAACACTTGGCATAGGC-3'	1168-1188
BPOLBO2	5’-TCTTGTCTTACTTTTGGAAGA-3'	2216-2236
POLSEQ4	5’-AAATGCCCCTATCTTATCAA-3'	2305-2323
LSOB1	5’-GGCATTATTTGCATACCCTTTGG-3'	2739-2762
POLSEQ5	5’-GGGTCCTTGTTGGGGTTGAAG-3'	2979-2999
B935	5’-GAAGGTTTGTGGCTCCTCTG-3'	1240-1260
BPOLEO1	5’-CTGAGAGTCCAAGAGTCCTCT-3'	1657-1677
MDN5	5’-AGGAGGCTGTAGGCATAAAT-3'	1774-1794
B936	5’-TGGAGGCTTGAACAGTAGGACAT-3’	1851-1874
MDC1	5’-TTGATAAGATAGGGGCATTTG-3'	2304-2324