Induction of HSF1 expression is associated with sporadic colorectal cancer

Table 1 Analysis of scanned density on gene arrays

	Ratio of cancer tissue	Ratio of cancer tissue
Gene	vs normal tissue	vs normal tissue
	(β-actin)1	(GAPDH)2
Egr-1	1.16	0.94
Hsf1	1.43	1.17
i κ B	1.23	1.00
Mdm2	0.98	0.80
Hsp27	1.58	1.28
Hsp90	1.31	1.06
iNOS	1.49	1.22

¹Ratio of cancer tissue versus normal tissue (β-actin): “Ratio to β-actin” from cancer/“Ratio to β-actin”from normal tissue;

²Ratio of cancer tissue versus normal tissue (GAPDH): “Ratio to GAPDH” from cancer/“Ratio to GAPDH”from normal tissue.

Table 2 Comparison of gene expression between normal and cancer tissue by RT-PCR

Patient	Dukes’	Ratio of cancer vs normal1
		HSF12	iNOS2	MDR12
1	C	0.87	0.78	0.78
2	B	2.13	2.57	0.64
15	B	1.34	1.17	0.23
21	A	1.68	0.81	1.71
29	A	1.96	1.32	1.02
33	C	1.71	0.72	0.64
34	B	3.44	1.04	2.08
38	B	1.78	1.17	0.87
40	B	2.25	0.35	0.25
45	B	2.07	0.44	1.50
48	A	1.96	0.60	0.81
52	B	1.75	1.83	0.35
71	D	0.91	3.55	1.31
79	C	1.11	0.39	0.99
82	C	0.79	2.33	0.29
84	C	1.53	1.07	1.04
85	C	2.25	6.49	0.70
95	C	1.64	0.88	0.40
99	C	1.28	3.79	0.61
100	A	1.70	0.62	0.16
104	A	57.47	2.77	0.47
105	B	1.54	4.53	0.55
106	C	4.52	4.97	0.25
113	C	2.08	0.17	0.17
117	C	1.84	2.49	0.87
118	B	1.43	4.45	2.08
122	C	1.78	0.57	0.25
125	C	4.14	12.24	1.73
128	B	0.79	1.31	0.08
130	C	1.07	0.73	0.73
131	B	2.11	2.66	1.61
132	C	0.73	1.55	1.20
142	C	2.44	6.00	0.52
151	B	2.16	1.06	4.31
153	C	1.20	0.62	0.37

¹Ratio of HSF1, iNOS or MDR1 to β-actin;

²[(HSF1, iNOS or MDR1)/β-actin, Cancer)]/[(HSF1, iNOS or MDR1)/β-actin, Normal)].