Heparanase mRNA expression and point mutation in hepatocellular carcinoma

Table 1 Relationship between the HPA expression and the clinicopathological parameters of HCC

Items	Number	HPA positive	HPA negative	P value
Size of tumor
> 5 cm	25	13	12	0.250
≤ 5 cm	8	3	5
Tumor capsule
Integrated	14	5	9	0.130
Disintegrated	19	11	8
AFP
Positive	21	12	9	0.125
Negative	12	4	8
HBsAg
Positive	24	12	12	0.292
Negative	9	4	5
Liver cirrhosis
Yes	18	8	10	0.241
No	15	8	7
Edmondson grade
I, II	13	3	10	0.019
III, IV	20	13	7
TNM staging
I, II	18	6	12	0.047
III, IV	15	10	5

Table 2 Relationship between HPA expression and metastatic recurrence of HCC

Items	Number	HPA positive	HPA negative	P value
Tendency to metastatic recurrence
High	14	10	4	0.023
Low	19	6	13
Metastatic recurrence
Yes	14	11	3	0.003
No	14	3	11

Table 3 Sequencing and corresponding series number

Number	Nucleotide sequence						Remarks
	TTGG	GCCCGACGTC	GCATGCTCCC	GGCCGCCATG	GCGGCCGCGG	GAATTCGATT	Plasmid DNA
409-468	TTCGATCCCA	AGAAGGAATC	AA1CCTTTGAA	GAGAGAAGTT	ACTGGCAATC	TCAAGTCAAC	HPA cDNA
469-528	CAGGATATTT	GCAAATATGG	ATCCATCCCT	CCTGATGTGG	AGGAA3,5AAGTT	ACGGTTGGAA	HPA cDNA
529-588	TGGCCCTACC	AGGAGCAATT	GCTACTCCGA	GAACACTACC	AGAAAAAGTT	CAAGAACAGC	HPA cDNA
589-648	ACCTACTCAA	GAAGCTCTGT	AGATGTGCTA	TACACTTTTG	CAAACTGCTC	AGGACTGGAC	HPA cDNA
649-708	TTGATCTTTG	GCCTAAATGC	GTTATTAAGA	ACAGCAGATT	TGCAGTGGAA	CAGTTCTAAT	HPA cDNA
709-768	GCTCAGTTGC	TCCTGGACTA	CTGCTCTTCC	AAGGGGTATA	ACATTTCTTG	GGAACTAGGC	HPA cDNA
769-828	AATGAACCTA	ACAGTTTCCT	TAAGAAGGCT	GATATTTTCA	TCAATGGGTC	GCAGTTAGGA	HPA cDNA
829-888	GAAGATTTTA	TTCAATTGCA	TAAACTTCTA	AGAAAGTCCA	CCTTCAAAAG4	T6GCAAAACTC	HPA cDNA
889-948	TATGGTCCTG	ATGTTGGTCA	GCCTCGAAGA	AAGACGGCTA	AGATGCTGAA	GAGCTTCCTG	HPA cDNA
949-993	AAGGCTGGTG	GAGAAGTGAT	TGATTCAGTT	ACATGGCATC	ACTAC2		HPA cDNA
	AATCACTAGT	GAATTCGCGG	CCGCCTGCAG	GTCGACCATA	TGGGAGAGCT	CCCAACGCGT	Plasmid DNA
	TGAATCACTA	GTGAATTCGC	GGCCGCCTGC	AGGTCGACCA	TATGGGAGAG	CTCCCAACGC	Plasmid DNA
	GTTG						Plasmid DNA

^1,2The segments with italics and boldface corresponded to the primers used in RT-PCR. ^3,4The letters represented the mutated bases. ^5,6The parts with underline represented the codons.