Inhibitory effect of ubiquitin-proteasome pathway on proliferation of esophageal carcinoma cells

Table 1 Evaluation of DNA synthesis by ³H-TdR incorporation (mean ± SD)

Group	24 h	48 h	72 h	96 h
Control MG-132 (μmol/L)	4295.52 ± 136.32	5236.17 ± 221.36	5642.92 ± 105.41	5863.43 ± 206.58
0.5	3764.68 ± 97.37a	2879.83 ± 86.25b	1918.73 ± 76.49b	1759.29 ± 89.23b
1.0	3526.14 ± 101.42a	2643.29 ± 79.38b	1547.25 ± 68.94b	1366.18 ± 52.49b
2.5	3402.34 ± 93.44a	2567.76 ± 68.21b	1260.37 ± 51.27b	910.25 ± 45.37b
5.0	3324.78 ± 65.43a	2411.56 ± 69.34b	840.79 ± 41.17b	517.83 ± 41.26b
10.0	3301.29 ± 59.28a	2360.40 ± 49.28b	820.56 ± 39.76b	498.71 ± 40.14b
15.0	3294.12 ± 67.33a	2324.25 ± 47.30b	810.17 ± 45.61b	485.26 ± 37.56b
20.0	3280.54 ± 62.46a	2320.60 ± 46.83b	804.63 ± 51.34b	476.90 ± 38.41b

^aP < 0.05,

^bP < 0.01 vs control.

Table 2 Effect of MG-132 on telomerase activity of EC9706 cells (mean ± SD)

Groups	Light absorption (A)
	24 h	48 h	72 h	96 h
Control	1.871 ± 0.061	2.234 ± 0.092	2.907 ± 0.113	3.025 ± 0.120
MG-132	0.154 ± 0.008b	0.085 ± 0.006b	0.072 ± 0.004b	0.067 ± 0.003b

^bP < 0.01 vs control group.

Table 3 Effect of MG-132 on cell cycle of EC9706 cells (mean ± SD, %)

Groups	48 h			96 h
	G0/G1	S	G2/M	G0/G1	S	G2/M
Control	40.4 ± 3.9	46.5 ± 4.3	13.1 ± 1.0	44.6 ± 4.1	40.9 ± 2.3	14.5 ± 1.4
MG-132	67.5 ± 5.1b	29.3 ± 2.8b	3.2 ± 0.2b	73.1 ± 5.2b	24.6 ± 2.1b	2.3 ± 0.1b

^bP < 0.01 vs control group.