Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines

Table 1 Sequence of primers and probes for real-time PCR

Gene	Primer (forward) (5’→3’)	Primer (reverse) (5’→3’)	Probe	GenBank accession number
p21WAF1	CTGGAGACTCTC	GGATTAGGGCTT	ACGGCGGCAGAC	NM_078467
	AGGGTCGAA	CCTCTTGGA	CAGCATGA
β-actin	CTGGCACCCAGC	GGACAGCGA	ATCATTGCT	BC016045
	ACAATG	GGCCAGGAT	CCTCCTGAG

Table 2 Sequence of primers and program of PCR for ChIPs

Primers	Sense (5’→3’)	Antisense (5’→3’)	Size of product and PCR condition	GenBank accession number
γ-actin	GGACCTGGC	GTGGCCATCTCC	153 bp 95 °C 5 min 95 °C 1 min,
	TGGCCGGGACC	TGCTCGAA	56 °C 1 min, 72 °C 1 min, 35 cycles
p21WAF1 (P1)	CGTGGTGGTGGT	CTGTCTGCA	296 bp 95 °C 5 min 95 °C 1 min,	U24170
	GAGCTAGA	CCTTCGCTCCT	58 °C 1 min, 72 °C 1 min, 35 cycles
p21WAF1 (P2)	GGTTGTATA	CTCTCACCTCCT	128 bp 95 °C 5 min 95 °C 1 min,	U24170
	TCAGGGCCG	CTGAGTGC	58 °C 1 min, 72 °C 1 min, 35 cycles