Simultaneous detection of HBV and HCV by multiplex PCR normalization

Table 1 Amplification results of HBV DNA from 20 HBsAg positive sera

Method	Sample number
	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20
ELISA	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+
Method 1	-	-	+	+	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-
Method 2	+	-	-	+	+	+	+	+	+	-	+	+	+	-	-	-	+	+	-	-
Method 3	+	+	+	+	+	+	+	+	+	-	-	+	+	+	+	-	+	+	+	+
Mrthod 4	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+

Note: + represents positive amplification; - represents negative amplification.

Table 2 Amplification results of HCV RNA from 15 anti-HCV positive sera

Method	Sample number
	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
ELISA	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
Method 1	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-
Method 2	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-
Method 3	-	+	+	+	-	-	-	-	+	+	-	-	+	-	-
Mrthod 4	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+

Note: + represents positive amplification; - represents negative amplification.

Table 3 Evaluation of validity and diagnostic power of nor-malized PCR for detecting HBV DNA

	HBV patient	Non-HBV control	Total
Positive	11	2	13
Negative	3	8	11
Total	14	10	24

Table 4 Evaluation of validity and diagnostic power of nor-malized PCR for detecting HCV RNA

	HCV patients	Non-HCV control	Total
Positive	6	1	7
Negative	2	9	11
Total	8	10	18

Table 5 Evaluation of validity and diagnostic power of nor-malized method for detecting HBV and HCV double infection

	HBV(+)and anti-HCV(+)	HBV(-) and anti-HCV(-)	Total
Positive	5	8	13
Negative	1	2	3
Total	6	10	16