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©The Author(s) 2004.
World J Gastroenterol. Jul 15, 2004; 10(14): 2055-2059
Published online Jul 15, 2004. doi: 10.3748/wjg.v10.i14.2055
Published online Jul 15, 2004. doi: 10.3748/wjg.v10.i14.2055
Table 1 Results of limulus lysate assay of H-LPS and E-LPS
Group | LPS (ng/mL) | |||||||
10.0 | 5.0 | 2.5 | 1.0 | 0.5 | 0.25 | 0.1 | 0.05 | |
H-LPS | + | + | + | + | + | - | - | - |
E-LPS | + | + | + | + | + | - | - | - |
Pyrogen-free water | negative in two repeated samples |
Table 2 PBMC number changes in the rabbits injected with H-LPS or E-LPS
Time (h) | PBMC (mean ± SD, × 109/L) | ||
H-LPS | E-LPS | Saline | |
Before injection | 4.52 ± 1.28 | 4.38 ± 1.35 | 4.05 ± 1.42 |
After injection | |||
0.5 | 12.51 ± 0.54 | 8.22 ± 0.68 | 4.25 ± 1.28 |
1.0 | 2.55 ± 0.87 | 2.23 ± 1.32 | 4.40 ± 1.19 |
1.5 | 1.56 ± 1.33 | 1.58 ± 1.27 | 4.08 ± 1.24 |
2.0 | 1.62 ± 0.93 | 1.64 ± 0.89 | 4.05 ± 1.06 |
2.5 | 1.65 ± 1.12 | 1.60 ± 1.34 | 4.35 ± 1.17 |
3.0 | 1.56 ± 1.45 | 1.52 ± 1.10 | 4.16 ± 1.22 |
Table 3 Cytotoxicity of H-LPS and E-LPS to mice fibroblast
Group | LPS (µg/mL) | CPM (mean ± SD) | t | P |
H-LPS | 1.0 | 7722 ± 819 | 3.74 | < 0.050 |
5.0 | 4724 ± 726 | 7.29 | < 0.010 | |
10.0 | 4328 ± 1194 | 6.56 | < 0.010 | |
50.0 | 3618 ± 434 | 9.27 | < 0.001 | |
100.0 | 2963 ± 764 | 9.20 | < 0.001 | |
E-LPS | 1.0 | 6813 ± 1183 | 4.17 | < 0.050 |
5.0 | 4290 ± 474 | 8.37 | < 0.005 | |
10.0 | 3516 ± 645 | 8.96 | < 0.001 | |
50.0 | 3224 ± 534 | 9.55 | < 0.001 | |
100.0 | 2513 ± 630 | 10.72 | < 0.001 | |
Control | 0 | 11083 ± 1324 |
Table 4 Toxicity test results in mice injected with H-LPS or E-LPS
Group | Number (n) | Dosageb (mg/mouse) | Death/Survival (n/n) | Mortality (%) |
H-LPS | 8 | 0.25 | 0/8 | 0 |
8 | 0.50 | 4/4 | 50.0 | |
8 | 1.00 | 6/2 | 75.0 | |
E-LPS | 8 | 0.25 | 2/6 | 25.0 |
8 | 0.50 | 7/1 | 87.5 | |
8 | 1.00 | 8/0 | 100 | |
8 | / | 0/8 | 0 |
Table 5 H-LPS detection rates in the biopsy specimens from patients with chronic gastritis and gastric ulcer
Group | Number (n) | Positivity (n) | Positive rate (%) |
Chronic gastritis | |||
Superficial | 48 | 38 | 79.2 |
Active | 10 | 9 | 90.0 |
Atrophic | 10 | 3 | 30.0 |
Gastric ulcer | |||
Gastric | 12 | 7 | 58.3 |
Duodenal | 40 | 16 | 40.0 |
Complex | 6 | 3 | 50.0 |
Total | 126 | 76 | 60.3 |
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Citation: Luo YH, Yan J, Mao YF.
Helicobacter pylori lipopolysaccharide: Biological activitiesin vitro andin vivo , pathological correlation to human chronic gastritis and peptic ulcer. World J Gastroenterol 2004; 10(14): 2055-2059 - URL: https://www.wjgnet.com/1007-9327/full/v10/i14/2055.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i14.2055