Expression of gonadotropin-releasing hormone receptor and effect of gonadotropin-releasing hormone analogue on proliferation of cultured gastric smooth muscle cells of rats

Table 1 Effects of alarelin on A value of MTT of rat GSMC

Group	MTT/A value (n = 12)
Control	0.728 ± 0.100
10-9 mol/L alarelin	0.533 ± 0.073
10-7 mol/L alarelin	0.368 ± 0.029
10-5 mol/L alarelin	0.243 ± 0.042

Table 2 Effects of alarelin on ³H-TdR Incorporation of rat GSMC

Group	3H-TdR Incorporation (n = 6)
Control	1 936.50 ± 440.99
10-9 mol/L alarelin	1 448.17 ± 327.72
10-7 mol/L alarelin	945.83 ± 374.32
10-5 mol/L alarelin	385.83 ± 184.66

Table 3 Effects of alarelin on average fluorescent values of PCNA in GSMC

Group	Average fluorescent values of PCNA (n = 5)
Control	13.48 ± 1.31
10-9 mol/L alarelin	11.37 ± 1.99
10-7 mol/L alarelin	9.35 ± 1.38
10-5 mol/L alarelin	7.37 ± 1.06

Table 4 Effects of alarelin on GSMC cell cycles (n = 4)

Group	Concentration of alarelin	G1 phase (%)	S phase (%)
Group I	Control	70.80 ± 11.30	17.50 ± 3.40
Group II	10-9 mol/L alarelin	80.00 ± 9.42	9.93 ± 1.73
Group III	10-8 mol/L alarelin	76.60 ± 8.99	7.10 ± 1.05
Group IV	10-7 mol/L alarelin	79.60 ± 7.43	6.60 ± 1.54
Group V	10-6 mol/L alarelin	78.50 ± 10.66	5.10 ± 1.01
Group VI	10-5 mol/L alarelin	80.20 ± 10.03	3.20 ± 1.20