Copyright
©The Author(s) 2004.
World J Gastroenterol. Jun 15, 2004; 10(12): 1746-1749
Published online Jun 15, 2004. doi: 10.3748/wjg.v10.i12.1746
Published online Jun 15, 2004. doi: 10.3748/wjg.v10.i12.1746
Table 1 Transient expression of pcDNA3.1(-)-core in HepG2 cells
| Group | Coat Ag | P | P/N (n = 0.05) | Results (+/-) |
| pcDNA3.1(-)-core | pcDNA3.1(-)-core lysate | 0.219 | 4.38 | + |
| Blank plasmid | pcDNA3.1(-) lysate | 0.034 | 0.68 | - |
| Positive control | HCV core Ag | 1.299 | 24.60 | + |
| Negative control | PBS | 0.012 | 0.24 | - |
Table 2 Comparison between positive clones and similar sequences in GenBank
| High similarity to known genes | Number of similar clones |
| Ribosomal protein | 21 |
| Eukaryotic translation elongation factor | 6 |
| Eukaryotic translation initiation factor | 5 |
| Heat shock factor binding protein (HSBP) | 5 |
| Apolipoprotein | 5 |
| NADH dehydrogenase | 3 |
| Heterogeneous nuclear ribonucleoprotein | 3 |
| D-like (HNRPDL), transcript variant | |
| Alpha-2-macroglobulin (A2M) | 2 |
| Bile acid-binding protein (BABP) | 2 |
| Cathepsin C (CTSC) | 1 |
| Fibronectin (FN precursor) | 1 |
| Magnesium-dependent protein phosphatase | 1 |
| Thioredoxin-related transmembrane protein | 1 |
| WEE1 gene for protein kinase | 1 |
- Citation: Liu M, Liu Y, Cheng J, Zhang SL, Wang L, Shao Q, Zhang J, Yang Q. Transactivating effect of hepatitis C virus core protein: A suppression subtractive hybridization study. World J Gastroenterol 2004; 10(12): 1746-1749
- URL: https://www.wjgnet.com/1007-9327/full/v10/i12/1746.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i12.1746