Differential gene expression between squamous cell carcinoma of esophageus and its normal epithelium; altered pattern of mal, akr1c2, and rab11a expression

Table 1 Sex, age and histopathological status of the inspected ESCC patients

No	Sex	Age	Differentiation degree	Metastatic
			of ESCC tumor	lymphnodes
1	F	47	Moderately differentiated	None
2	M	53	Well differentiated	3 out of 8
3	F	47	Well differentiated	2 out of 10
4	F	50	Moderately differentiated	1 out of 1
5	M	69	Moderately differentiated	3 out of 9
6	F	60	Moderately differentiated	1 out of 6
7	M	66	Moderately differentiated	All five
8	M	76	Moderately differentiated	None
9	F	55	Poorly differentiated	No evidence
10	F	70	Well differentiated	All three

Table 2 The cDNAs, primers have been used in RT reactions and their related genes with different expression level in normal esophageal and ESCC tissues

cDNA name	cDNA’s length (bp)	3' end primer (5'→3')	5' end primer (5'→3')	GeneBank accession number
ESC1	360	AAG CTT TTT TTT TTT G	AAG CTT TAC GTA C	BC000458
ESC2	273	AAG CTT TTT TTT TTT A	AAG CTT CTC AGA C	BC013348
ESC3	300	AAG CTT TTT TTT TTT C	AAG CTT CTC AGA C	BC007024
ESC4	325	AAG CTT TTT TTT TTT C	AAG CTT AAC GAGG	BC006095
ESC5	442	AAG CTT TTT TTT TTT G	AAG CTT GAT TCG C	BC000072
ESC6	271	AAG CTT TTT TTT TTT C	AAG CTT GCG ATG T	AF_061732
ESC7	273	AAG CTT TTT TTT TTT C	AAG CTT TGG TCA G	NM001759