A rapid and efficient method to express target genes in mammalian cells by baculovirus

Table 1 Comparison of gene-transfer and expression efficiency in HepG2 and CV1 cells among different gene-transfer systems

	Gene-transfer rate (%)		Mean fluorescence intensity
	HepG2	CV1	HepG2	CV1
LipofectAMINE	29.6	34.2	138.1	103.4
Retro-EGFP	13.5	18.2	95.8	76.6
Vaccinia-EGFP	93.5a	94.2a	172.5a	148.41
BacV-CMV-EGFPA	92.5	95.6	294.5	232.1

¹Cytopathic effects could be observed within 24 h post infec-tion and most cells were lysised within 48 h.

Table 2 Comparison of gene-transfer and expression efficiencies in different mammalian cell lines transfected with pcDNA3.1-EGFP

Organism		Tissue	Growth properties	Transfection efficiency (%)	Mean fluorescence intensity
Human
	WI-38	Lung fibroblast	Adherent	24.53	58.3
	HepG2	Hepatocellular carcinoma	Adherent	18.60	79.4
	Hela	Adenocarcinoma	Adherent	19.82	42.7
	293	Kidney	Adherent	61.45	86.6
	Raji	Burkitt’s lymphoma	Suspension	5.56	14.6
	LCL-cm	B lymphocyte	Suspension	7.62	17.4
Mouse
	BNL 1ME A.7R.1	Liver	Adherent	13.09	19.1
	CHO-K1	Ovary	Adherent	47.36	48.6
	JC	Adenocarcinoma	Adherent	12.54	22.8
	PT67	Embryo fibroblast	Adherent	22.63	31.6
	NIH3T3	Embryo fibroblast	Adherent	25.31	35.3
	P815	Mastocytoma	Suspension	12.78	16.8

Table 3 Comparison of transduction efficiencies in adherent and suspended culture cells of different species transduced with BacV-CMV-EGFPA

		Adherent culture	Suspended culture
Primate	Total	10	3
	Mean transduction efficiency (%)	77.25 ± 11.37	1.12 ± 0.53
Mice	Total	6	1
	Mean transduction efficiency (%)	21.84 ± 15.85	0.61